Activity and transcriptional regulatory elements of the promoter in Arctic fox (Vulpes lagopus) β-defensin103 gene.

Min Guo; Ziya Zhao; Ruining Wang; Xiaoning Zheng; Yongdong Peng; Zhengzhu Liu; Xianglong LI; Yuanfang Gong

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Activity and transcriptional regulatory elements of the promoter in Arctic fox (Vulpes lagopus) β-defensin103 gene.

Author: Min GUO ¹ ; Ziya ZHAO ¹ ; Ruining WANG ¹ ; Xiaoning ZHENG ² ; Yongdong PENG ¹ ; Zhengzhu LIU ¹ ; Xianglong LI ¹ ; Yuanfang GONG ¹
Author Information

1. College of Animal Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao 066004, Hebei, China.
2. College of Animal Science and Technology, Agricultural University of Hebei Province, Baoding 071000, Hebei, China.
Publication Type:Journal Article
Keywords: Arctic fox; CBD103 gene; promoter activity; transcriptional regulatory element
MeSH: Animals; Binding Sites; Foxes; Luciferases; Promoter Regions, Genetic; Sp1 Transcription Factor; beta-Defensins
From: Chinese Journal of Biotechnology 2019;35(8):1469-1477
CountryChina
Language:Chinese
Abstract: The aim of this study was to screen the active regions and transcription factor binding sites in the promoter of the CBD103 gene related to Arctic fox coat color, and to provide a basis for revealing the molecular genetic mechanism of CBD103 gene regulating the coat color formation. The 5'-flanking region fragment 2 123 bp of Arctic fox CBD103 gene was cloned, and 4 truncated promoter reporter vectors of different lengths were constructed. The promoter activity was detected by the dual-luciferase reporter assay system. Point mutations were performed on the 3 predicted specificity protein 1 (Sp1) transcription factor binding sites in the highest promoter active region, and 3 mutant vectors were constructed. The activity was then detected by the dual-luciferase reporter assay system. The results showed that the region 1 656 (-1 604/+51) had the highest activity in the 4 truncated promoters of different lengths, and the promoter activity of the three mutant vectors constructed in this region were significantly lower than that of the wild type (fragment 1 656). The region of -1 604 /+51 was the core promoter region of CBD103 gene in Arctic fox and -1 552/-1 564, -1 439/-1 454 and -329/-339 regions were positive regulatory regions. This study successfully obtained the core promoter region and positive regulation regions of the Arctic fox CBD103 gene, which laid a foundation for further study on the molecular genetic mechanism of this gene regulating Arctic fox coat color.