Catalysis of enzymes under industrial environment and their adaptive modifications: a review.

Author: Wenhao WANG ¹ ; Pengfei WEN ¹ ; Kongliang XU ¹ ; Renchao ZHENG ¹ ; Yuguo ZHENG ¹
Author Information

1. Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, Zhejiang, China.
Publication Type:Journal Article
Keywords: adaptive modification; biocatalysis; catalysis; enzymes; industrial environment
MeSH: Biocatalysis; drug effects; Biotechnology; Enzymes; chemistry; metabolism; Hot Temperature; Hydrogen-Ion Concentration; Protein Engineering; Solvents; chemistry; pharmacology
From: Chinese Journal of Biotechnology 2019;35(10):1857-1869
CountryChina
Language:Chinese
Abstract: Enzymes have a wide range of applications and great industrial potential. However, large-scale applications of enzymes are restricted by the harsh industrial environment, such as high temperature, strong acid/alkali, high salt, organic solvents, and high substrate concentration. Adaptive modification (such as rational or semi-rational design, directed evolution and immobilization) is the most common strategy to improve the catalysis of enzymes under industrial conditions. Here, we review the catalysis of enzymes in the industrial environment and various methods adopted for the adaptive modifications in recent years, to provide reference for the adaptive modifications of enzymes.