Flavor and meridian tropism classification analysis of Callianthemum taipaicum.
10.19540/j.cnki.cjcmm.20171027.016
- Author:
Yan-Ru LIU
1
;
Zhi-Shu TANG
1
;
Jin-Ao DUAN
2
;
Xiao-Chun SUN
1
;
Zhong-Xing SONG
1
;
Xin-Bo SHI
1
;
Yang LV
1
;
Zhao ZHANG
1
;
Da-Wei QIAN
2
Author Information
1. Shaanxi Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Shaanxi Province Key Laboratory of New Drugs and Chinese Medicine Foundation Research, Shaanxi University of Chinese Medicine, Xianyang 712083, China.
2. Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Jiangsu Key Laboratory for High Technology Research of Traditional Chinese Medicine Formulae, and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China.
- Publication Type:Journal Article
- Keywords:
Callianthemum taipaicum;
Ranunculaceae;
liquid chromatography-tandem mass spectrometry;
meridian tropism of Chinese medicinal herbs;
partial least squares-discriiminate analysis
- MeSH:
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal;
chemistry;
Mass Spectrometry;
Meridians;
Phytochemicals;
analysis;
Plants, Medicinal;
chemistry;
Ranunculaceae;
chemistry;
Tandem Mass Spectrometry
- From:
China Journal of Chinese Materia Medica
2018;43(2):353-362
- CountryChina
- Language:Chinese
-
Abstract:
To explore the flavor and meridian tropism classification of Callianthemum taipaicum by principal components analysis(PCA) and partial least square analysis(PLS). Meanwhile,to establish a high performance liquid chromatography-tandem mass spectrometry(HPLC-ESI-MS) method for the simultaneous determination of 55 active components from 13 kinds of Ranunculaceae of Chinese traditional herbs. Samples were separated on HPLC system by Agilent 5 TC-C₁₈（2）（4.6 mm×250 mm，5 μm）column and eluted with acetonitrile and 0.1% formic acid at the flow rate of 0.6 mL·min⁻¹. The data were performed by HPLC-ESI-MS with multiple reaction monitoring（MRM）scanning mode under positive and negative ion modes and quantified by external standards. The data from 13 Ranunculaceae herbs were analyzed by the PLS-tree and cooman's prediction of PCA and PLS to evaluate the similarities and differences of C. taipaicum in flavor and meridian tropism. The results showed that calibration curves of 55 components all showed good linearity, >0.99,with good precision, repeatability and stability. After compared to other 12 herbs,PCA and PLS results revealed that the C. taipaicum belonged to lung and bladder meridians while its flavor attributive to pungent,warm in nature. In conclusion,the analysis approach of chemometric calculation combined with multi-components quantification is suitable for the classification of meridian tropism and flavor of Chinese traditional medicine,which can be used for alternative research of rare herbs.
