Influence of light on gene expression of key synthesis enzyme genes FtANR and FtLAR about proanthocyanidin in seeds of homologous plant of food and medicine Fagopyrum tataricum.
10.19540/j.cnki.cjcmm.20180105.005
- Author:
Chun-Li JIANG
1
;
Xin-Yao SU
1
;
Ya-Chun XU
1
;
Er-Yi WU
2
;
Yu-Hua SHI
3
;
Dong ZHANG
3
;
Qing-Fu CHEN
4
;
Wei SUN
3
;
Jian-Ping XUE
1
Author Information
1. College of Life Sciences， Huaibei Normal University， Huaibei 235000， China.
2. College of Life Science， Northwest Agriculture & Forestry Unversity， Yangling 712100， China.
3. Key Laboratory of Beijing for Identification and Safe Evaluation of Chinese Medicine， Institute of Chinese Meteria Medica， China Academy of Chinese Medical Sciences， Beijing 100700， China.
4. Guizhou Normal University， Guiyang 550000， China.
- Publication Type:Journal Article
- Keywords:
Fagopyrum tataricum;
gene expression;
light induction;
proanthocyanidin;
quantitative analysis
- MeSH:
Fagopyrum;
enzymology;
radiation effects;
Gene Expression Regulation, Plant;
radiation effects;
Light;
NADH, NADPH Oxidoreductases;
genetics;
Plant Proteins;
genetics;
Proanthocyanidins;
biosynthesis;
Seeds;
enzymology;
radiation effects
- From:
China Journal of Chinese Materia Medica
2018;43(3):469-477
- CountryChina
- Language:Chinese
-
Abstract:
Tartary buckwheat Fagopyrum tataricum is an important medicinal and functional herb due to its rich content of flavonoids in the seeds. F.tataricum exhibited good functions for free radicals scavenging， anti-oxidation， anti-aging activities. Although much genetic knowledge of the synthesis， regulation， accumulation of rutin， the genetic basis of proanthocyanidins(PAs) in tartary buckwheat and their related gene expression changes under different lights(blue， red， far red， ultraviolet light) remain largely unexplored. In this study， we cloned one anthocyanidin reductase gene(ANR) and two leucocyanidin reductase gene(LAR) named FtANR，FtLAR1，FtLAR3 involved in formation of(+)-catechin and(-)-epicatechin precusor proanthocyanidin by digging out F. tataricum seed transcriptome data. The expression data showed that the opposite influence of red light on these gene transcript level compared to others lights. The expression levels of FtANR and FtLAR1 decreased and FtLAR3 appeared increment after exposed in the red light， while the expression levels of those genes appeared opposite result after exposed in the blue and far red light.
