Identification of endophytic bacteria BZJN1 and research on biological control of root rot of Atractylodes macrocephala.
10.19540/j.cnki.cjcmm.20180105.008
- Author:
Jing-Mao YOU
1
;
Kun XIONG
2
;
Sen MU
1
;
Jie GUO
1
;
Xiao-Liang GUO
1
;
Yuan-Yuan DUAN
1
;
Juan LI
1
;
Fan CAO
1
;
Zong-Cheng ZOU
3
;
Hai TAN
3
Author Information
1. Institute of Chinese Herbal Medicines， Hubei Academy of Agricultural Sciences， Enshi 445000， China.
2. Enshi for Food and Drug Inspection and Testing Center， Enshi 445000， China.
3. Ba Dong Jin Da Pharmaceutical Co.， Ltd.， Enshi 445000， China.
- Publication Type:Journal Article
- Keywords:
Atractylodes macrocephala;
Bacillus subtilis;
Ceratobasidium sp;
control
- MeSH:
Atractylodes;
microbiology;
Bacillus subtilis;
physiology;
Basidiomycota;
pathogenicity;
Biological Control Agents;
Endophytes;
classification;
isolation & purification;
Plant Diseases;
microbiology;
prevention & control
- From:
China Journal of Chinese Materia Medica
2018;43(3):478-483
- CountryChina
- Language:Chinese
-
Abstract:
In this study， an endophytic bacteria strain BZJN1 was isolated from Atractylodes macrocephala， and identified as Bacillus subtilis by physiological and biochemical tests and molecular identification. Strain BZJN1 could inhibit the growth of mycelia of Ceratobasidium sp. significantly， and the inhibition rate was more than 70%. The mycelium growth deformity with bulge as spherical and partially exhaustible in apex or central with microscopic observation. The inhibitory rates under 3% and 6% concentrations of the cell free fermentation were 22.7% and 38.7% expectively. The field test proved that the control efficacy of treatment of 1×10⁸ cfu·mL⁻¹ is 75.27% and 72.37% after 10 and 20 days. All the treatments of strain BZJN1 was able to promote the growth of A. macrocephala， the treatment of 1×10⁸ cfu·mL⁻¹ could able to increase the yield to 14.1%.
