Identification and analysis of NAC family in ginger(Zingiber officinale) based on RNA-seq data.
10.19540/j.cnki.cjcmm.20180105.007
- Author:
Qin-Hong LIAO
1
;
Youg ZOU
1
;
Hong-Lei LI
1
;
Yu-Song JIANG
1
Author Information
1. Research Institute for Special Plants， Chongqing University of Arts and Sciences， Chongqing 402160， China.
- Publication Type:Journal Article
- Keywords:
NAC transcription factor;
RNA-seq;
gene differential expression;
ginger
- MeSH:
Gene Expression Regulation, Plant;
Ginger;
genetics;
Multigene Family;
Phylogeny;
Plant Proteins;
genetics;
Protein Structure, Tertiary;
RNA, Plant;
genetics;
Sequence Analysis, RNA;
Transcription Factors;
genetics
- From:
China Journal of Chinese Materia Medica
2018;43(3):493-501
- CountryChina
- Language:Chinese
-
Abstract:
The NAC family is an important transcription factor which regulate plant growth and development， signal transduction， and stress response.In this study， the protein identification， subfamily classification， the determination of physical and chemical properties， protein structure， and expression pattern of NAC family were performed using bioinformatic methods based on the RNA-seq data of ginger. The results showed that a total of 72 NAC transcription factors were identified in 271.1 Mb total nucleotides， and they could be clustered into 13 subfamilies according to the phylogenetic tree.The physical and chemical properties， structure analysis revealed that the amino acid number and isoelectric point were different among 13 NAC subfamilies; the secondary structure of NACs transcription factors mainly consist of random coil， and the tertiary structure is similar.In addition，the expression patterns of genes under different soil moisture and Ralstonia solanacearum infection showed that 23 NACs were differentially expressed， which were mainly distributed in Ⅷ，Ⅶ， and ⅩⅤ subfamilies related to plant senescence， hormone metabolism and cell wall metabolism.The results provide some valuable information for the research and development of NAC transcription factors in ginger.
