Comparison of saponins from Gynostemma pentaphyllum leaves prepared by different processing methods.
10.19540/j.cnki.cjcmm.20171218.001
- Author:
Li-Hua DONG
1
;
Yan-Hui KUANG
2
;
Dong-Dong FAN
2
;
Tong JIANG
3
;
Liang-Mian CHEN
3
;
Dong ZHANG
3
;
Jing-Jing ZHU
3
;
Zhi-Min WANG
3
;
De-Qin WANG
2
;
Chu-Yuan LI
2
Author Information
1. Henan University of Traditional Chinese Medicine， Zhengzhou 450008， China.
2. Hutchison Whampoa Guangzhou Baiyunshan Chinese Medicine Co.， Ltd.， Guangzhou 510515， China.
3. National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines， Institute of Chinese Materia Medica， China Academy of Chinese Medical Sciences， Beijing 100700， China.
- Publication Type:Journal Article
- Keywords:
Gynostemma pentaphyllum;
different processing methods;
mass spectrometry identification;
saponins
- MeSH:
Chromatography, High Pressure Liquid;
Gynostemma;
chemistry;
Mass Spectrometry;
Plant Leaves;
chemistry;
Saponins;
chemistry;
isolation & purification
- From:
China Journal of Chinese Materia Medica
2018;43(3):502-510
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the differences of chemical compositions in Gynostemma pentaphyllum leaves prepared by different processing methods. Ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS) was used to compare the chemical compositions between shade-dried processing and drum-dried processing. Forty six gypenosides were identified by control comparison， liquid chromatography-mass spectrometry(LC-MSn) fragmentation information， and literature data. The mass spectral peak area statistics was combined with principal component analysis(PCA)， and the results showed that eight batches of Gynostemma pentaphyllum leaves samples were divided into two groups according to the two different processing methods; ten chemical compositions with significant differences were screened according to mass spectrum information combined with partial least-squares discriminant analysis(PLS-DA). The result showed that most parent nucleus of the gypenosides contained three to four glycosides in drum-dried samples， and one to two glycosides in the shade-dried samples. It was inferred from further MS analysis that desugarization of gypenosides was present to produce secondary glycosides with the effect of glucosidase in the shade-drying， thus resulting in difference in compositions. This study provided data support for harvesting， processing and quality control of Gynostemma pentaphyllum leaves.
