Limonoids from seeds of Azadirachta indica and their cytotoxic activity.
10.19540/j.cnki.cjcmm.20180104.010
- Author:
Xiao-Feng LU
1
;
Dong-Mei DAI
2
;
Rong-Min YU
1
;
Li-Yan SONG
1
;
Jian-Hua ZHU
1
;
Xiao-Na FAN
3
;
Jia-Chen ZI
1
Author Information
1. Biotechnological Institute of Chinese Materia Medica, Jinan University, Guangzhou 510632, China.
2. School of Biological and Chemical Engineering, Ningbo Institute of Technology, Zhejiang University, Ningbo 315100, China.
3. School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, China.
- Publication Type:Journal Article
- Keywords:
Azadirachta indica;
cytotoxic activity;
limonoids
- MeSH:
Azadirachta;
chemistry;
HL-60 Cells;
HeLa Cells;
Humans;
Limonins;
isolation & purification;
pharmacology;
Phytochemicals;
isolation & purification;
pharmacology;
Plant Extracts;
Seeds;
chemistry
- From:
China Journal of Chinese Materia Medica
2018;43(3):537-543
- CountryChina
- Language:Chinese
-
Abstract:
Eight limonoids were isolated from 95% ethanol extracts of neem(Azadirachta indica) seeds by various chromatographic methods. By comparison of their spectroscopic data with those reported in the literatures, these limonoids were determined as salannin(1), 1-detigloyl-1-isobutylsalannin(2), salannol-3-acetate(3), salannol(4), spirosendan(5), 1-detigloyloxy-3-deacetylsalannin-1-en-3-one(6), nimbin(7) and 6-deacetylnimbin(8). Compounds 2 and 5 were firstly isolated from this genus and 5 represented the only example of its type. And 6 is a new natural product. 6 showed inhibitory activity against HeLa and HL-60 cells, with IC₅₀ of(21.61±4.37) and(27.33±5.74) μmol·L⁻¹, respectively. Both 7 and 8 mildly inhibited the growth of HeLa cells, with IC₅₀ of (33.15±5.24) and (38.56±6.41) μmol·L⁻¹, respectively.
