Feasibility study of QAMS for quantitative analysis of multiple structural types of ingredients in Atractylodis Rhizome by GC.
10.19540/j.cnki.cjcmm.20180104.011
- Author:
Wen-Ting CHEN
1
;
Yang CHEN
1
;
Bo CUI
1
;
Xiao-Qi LI
1
;
Yan-Hao FAN
1
;
Xin-Hong WANG
1
;
Rui AN
1
Author Information
1. Shanghai University of Traditional Chinese Medicine， Shanghai 201203， China.
- Publication Type:Journal Article
- Keywords:
Atractylodis Rhizome;
GC;
QAMS;
relative correction factor(RCF);
sesquiterpene
- MeSH:
Atractylodes;
chemistry;
Chromatography, Gas;
Drugs, Chinese Herbal;
chemistry;
Feasibility Studies;
Phytochemicals;
analysis;
Reproducibility of Results;
Rhizome;
chemistry
- From:
China Journal of Chinese Materia Medica
2018;43(3):551-556
- CountryChina
- Language:Chinese
-
Abstract:
In this study， quantitative analysis of multi-components with single marker(QAMS) was established and validated to simultaneously determine four sesquiterpenoids(β-eudesmol， atractylon， atractylolideⅠ， atractylolide Ⅱ) in Atractylodis Rhizome based on the gas chromatographic method(GC). Using β-eudesmol as the contrast， the relative correctionfactors(RCF) of the other three sesquiterpenoids were determined by GC. Within the line arranges，the values of RCF of β-eudesmol to atractylon， atractylolideⅠand atractylolide Ⅱ were 0.823， 0.690 and 0.766， respectively. The RCF had a good reproducibility in various instruments， chromatographic columns. According to their RCF， we simultaneously determined four sesquiterpenoids in Atractylodis Rhizome only using one marker. The results of QAMS method were validated by comparing with that of internal standard method， and no obvious significant difference was found.
