Effects of Jinwu Jiangu recipe on IL-17/STAT3 signals in rheumatoid arthritis synoviocytes.
10.19540/j.cnki.cjcmm.20171113.008
- Author:
Wu-Kai MA
1
;
Rong LI
1
;
Qiao-Yi NING
1
;
Ying HUANG
1
;
Fang TANG
1
;
Dao-Min LU
1
;
Xue-Ming YAO
1
Author Information
1. Department of Rheumatology and Immunology， the Second Affiliated Hospital of Guiyang of Traditional Chinese Medicine， Guiyang 550003， China.
- Publication Type:Journal Article
- Keywords:
IL-17;
Jinwu Jiangu recipe;
Miao medicine;
rheumatoid arthritis;
signal transducer and activator of transcription 3;
synoviocytes
- MeSH:
Arthritis, Rheumatoid;
Cells, Cultured;
Drugs, Chinese Herbal;
pharmacology;
Fibroblasts;
Gene Expression Regulation;
Humans;
Nuclear Receptor Subfamily 1, Group F, Member 1;
metabolism;
Nuclear Receptor Subfamily 1, Group F, Member 3;
metabolism;
Receptors, Interleukin-17;
metabolism;
STAT3 Transcription Factor;
metabolism;
Synovial Membrane;
Synoviocytes;
drug effects
- From:
China Journal of Chinese Materia Medica
2018;43(3):585-590
- CountryChina
- Language:Chinese
-
Abstract:
This paper aimed to investigate the effects of Jinwu Jiangu recipe total extract on the IL-17/STAT3 signals in rheumatoid arthritis synovial fibroblasts(RASF). The primary RASFs were cultured by tissue piece method ， and divided into blank control group， Jinwu Jiangu recipe low dose group， Jinwu Jiangu recipe middle dose group， Jinwu Jiangu recipe high dose group， and tripterygium glycosides control group. They were then treated with corresponding serum free medium， different doses of Jinwu Jiangu recipe total extract(0.06， 0.6， 6.0 g·L⁻¹)， and tripterygium glycosides(0.03 g·L⁻¹) respectively for 24 hours. The gene expression levels of RORα， RORγt， and STAT3 mRNA were detected by polymerase chain reaction(PCR)， and the protein activity of IL-17R and pSTAT3 were measured by Western blot assay. The results showed that as compared with blank control group， the expression levels of RORα， RORγt， IL-17R and STAT3 mRNA in RASF were significantly declined(<0.01). As compared with tripterygium glycosides control group， Jinwu Jiangu recipe total extract middle dose group and high dose group can down-regulate the expression levels of RORα， RORγt， IL-17R and STAT3 mRNA(<0.05)， and the effect was more obvious in high dose group(<0.01). As compared with blank control group， the protein expression levels of IL-17R and pSTAT3 in each treatment group were obviously decreased(<0.01). As compared with tripterygium glycosides control group， Jinwu Jiangu recipe high dose group had more obvious effect in down-regulating the protein expression of pSTAT3(<0.01). Therefore， Miao medicine Jinwu Jiangu recipe total extract can down-regulate the expressions of RORα， RORγt， and STAT3 mRNA， and inhibit the protein activity of IL-17R and pSTAT3 in RASF.
