Absorption of Inula cappa extract based on everted intestinal sac method.
- Author:
Zi-Peng GONG
1
;
Mei LI
1
;
Jing-Yu HOU
1
;
Lin-Lin WU
1
;
Ting-Ting CHEN
1
;
Yue-Ting LI
1
;
Si-Ying CHEN
1
;
Yong-Jun LI
1
;
Ai-Min WANG
1
;
Yan-Yu LAN
1
;
Yong-Lin WANG
1
Author Information
- Publication Type:Journal Article
- Keywords: Inula cappa extract; absorption; dicaffeoylquinic acid; everted intestinal sac method
- MeSH: Animals; Chromatography, High Pressure Liquid; Intestinal Absorption; Intestines; drug effects; Inula; chemistry; Plant Extracts; pharmacology; Rats; Rats, Sprague-Dawley; Tandem Mass Spectrometry
- From: China Journal of Chinese Materia Medica 2018;43(3):609-617
- CountryChina
- Language:Chinese
- Abstract: To investigate the absorptive characteristics of Inula cappa extract based on the rat everted intestinal sac method . Nine representative ingredients in I. cappa extract were selected as the study objects. An UPLC-MS/MS method was established to determine and detect their cumulative absorption amount for expounding the absorptive characteristics of ingredients in different intestinal sections. According to the results， the transport mechanism of 8 compounds showed passive diffusion by the reverted gut sac method. And scopolin was actively transported in the intestine. The best absorption site of chlorogenic acid was duodenum. The best absorption site of cryptochlorogenic acid， 1，3--dicaffeoylquinic acid， luteolin-7-glucoside and 3，4--dicaffeoylquinic acid were jejunum. The best absorption site of neochlorogenic acid， scopolin， 4，5--dicaffeoylquinic acid and 3，5--dicaffeoylquinic acid was ileum. The absorption of all the compounds was affected by pH and bile. All of the nine ingredients in I. cappa extract could be absorbed in intestines， but with differences in the absorption rate， the best absorptive site and mechanism， indicating that the intestinal absorption of I. cappa extract was selective.
