Identification of bear bile powder and its adulterants by using DNA barcoding technology.
10.19540/j.cnki.cjcmm.2018.0014
- Author:
Ya-Chun XU
1
;
Chao XIONG
2
;
Chun-Li JIANG
1
;
Yong-Bo DUAN
1
;
Wei SUN
2
;
Shao-Yong LIU
3
;
Dong-Sheng XUE
3
;
Jian-Ping XUE
1
Author Information
1. School of Life Sciences, Huaibei Normal University, Huaibei 235000, China.
2. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
3. Shanghai Kaibao Pharmaceutical Co．, Ltd., Shanghai 201401, China.
- Publication Type:Journal Article
- Keywords:
COI sequence;
DNA barcoding;
adulterant molecular identification;
bear bile powder
- MeSH:
Animals;
Bile;
chemistry;
DNA Barcoding, Taxonomic;
Medicine, Chinese Traditional;
Phylogeny;
Quality Control;
Ursidae
- From:
China Journal of Chinese Materia Medica
2018;43(4):645-650
- CountryChina
- Language:Chinese
-
Abstract:
To identify the precious bile powder and its adulterants by DNA barcoding, and establish its standard experimental process to ensure the safe and effective utilization. Total twelve sequences from samples of bear bile powder which come from Ursus thibetanus for DNA extraction, PCR(polymerase chain reaction) and sequence, then using CodonCode Aligner V 7.0.1 shear primer region to obtain COI sequence. The COI sequences of U. arctos and their adulterants were obtained from GenBank. MEGA7.0 software was applied for analyzing mutation, calculating intraspecific and interspecific K2P(Kimura 2-Parameter) genetic distance and constructing the Neighbor-joining tree(NJ). The results showed that the maximum K2P genetic distance of bear bile powder of U. thibetanus and U. arctos are far less than minimum K2P genetic distance within its adulterants species, and the results of NJ tree demonstrated that each species could be distinguished from the counterfeits obviously. DNA barcoding is a safe, convenient and reliable technique for species identification, and it is important to establish the standard sequence of COI sequences for animal medicines.
