Efficient discovery and capturing of nNOS-PSD-95 uncouplers from Trifolium pratense.
10.19540/j.cnki.cjcmm.20171208.001
- Author:
Hong-Liang HE
1
;
Lin-Li PAN
2
;
Xiao-Li GU
3
;
Jiao-Jiao HUANG
2
;
Cheng-Hong SUN
2
;
Yu-Lin TANG
1
;
Li-Na CHEN
2
Author Information
1. Department of Clinical Pharmacy, Sir Run Run Hospital, Nanjing Medical University, Nanjing 211166, China.
2. School of Pharmacy, Nanjing Medical University, Nanjing 211166, China.
3. Nantong First People's Hospital, Nantong 226001, China.
- Publication Type:Journal Article
- Keywords:
Trifolium pratense;
ZL006;
efficient discovery;
magnetic molecularly imprinted polymers;
nNOS-PSD-95 uncouplers;
stroke
- MeSH:
Adsorption;
Genistein;
chemistry;
Molecular Imprinting;
Phytochemicals;
chemistry;
Polymers;
chemistry;
Solid Phase Extraction;
Spectroscopy, Fourier Transform Infrared;
Trifolium;
chemistry
- From:
China Journal of Chinese Materia Medica
2018;43(4):748-754
- CountryChina
- Language:Chinese
-
Abstract:
Magnetic molecularly imprinted polymers(MMIPs) were prepared with ZL006 as template, acrylamide(AA) as the functional monomer, and acetonitrile as pore-forming agent; then Fourier transform infrared spectroscopy(FT-IR) and scanning electron microscopy(SEM) were used to characterize their forms and structures. Simultaneously, the MMIPs prepared previously were used as sorbents for dispersive magnetic solid phase extraction(DSPE) to capture and identify potential nNOS-PSD-95 uncouplers from extracts of Trifolium pratense and the the activities of the screened compounds were evaluated by the neuroprotective effect and co-immunoprecipitation test. The experiment revealed that the successfully synthesized MMIPs showed good dispersiveness, suitable particle size and good adsorption properties. Formononetin, prunetin and biochanin A were separated and enriched from Trifolium pratense by using the MMIPs as artificial antibodies and finally biochanin A was found to have higher cytoprotective action and uncoupling action according to the neuroprotective effect and co-immunoprecipitation test.
