Protective effect of Dendrobium candidum on isoproterenol induced cardiac hypertrophy in rats.
10.19540/j.cnki.cjcmm.2018.0021
- Author:
Xiao-Chun XIAO
1
;
Wei-Hong CHEN
1
;
Yuan-Yuan CAO
2
;
Yong-Jun LOU
1
;
Yuan LIU
2
;
Jin WANG
2
;
Xu-Feng ZHAI
1
;
Nian-Sheng LI
2
;
Ying LI
3
Author Information
1. Infinitus(China) Company Ltd., Guangzhou 510663, China.
2. Department of Pharmacology, School of Pharmaceutical Science, Central South University, Changsha 410013, China.
3. Department of Health Administration, the Third Xiangya Hospital, Central South University, Changsha 410023, China.
- Publication Type:Journal Article
- Keywords:
ANP;
BNP;
Dendrobium candidum;
cTN-I;
myocardial hypertrophy
- MeSH:
Animals;
Cardiomegaly;
drug therapy;
Dendrobium;
chemistry;
Drugs, Chinese Herbal;
pharmacology;
Female;
Isoproterenol;
Male;
Myocardium;
pathology;
Rats;
Rats, Sprague-Dawley
- From:
China Journal of Chinese Materia Medica
2018;43(4):800-804
- CountryChina
- Language:Chinese
-
Abstract:
To study the effect and mechanism of Dendrobium candidum on isoproterenol-induced myocardial hypertrophy in rats, 60 healthy SD rats(30 males and 30 females) were randomly divided into 5 groups(12 in each group): normal group, model group, three D. candidum preventive administration groups(0.09, 0.18, 1.1 g·kg⁻¹). Except for the normal group, rats of other groups were injected back subcutaneously with ISO(5 mg·kg⁻¹) for 10 consecutive days. At the same time, preventive administration groups began to give different doses of the sample for 30 days and model group began to give normal saline. Left ventricular systolic pressure(LVSP) was measured in each group by common carotid artery cannulation, and the left ventricle(LW)/tibia length, heart weight index(HWI) and myocardial hydroxyproline(Hydro) content were calculated. Myocardial tissue HE staining and Masson staining were used to observe the myocardial structure and the degree of myocardial fibrosis respectively. Atrial natriuretic peptide(ANP), brain natriuretic peptide(BNP), and cardiac troponin I(cTN-I) concentration were measured by enzyme-linked immunosorbent assay(ELISA). The results showed that as compared with the normal group, the levels of ANP, BNP and cTN-I in plasma were significantly increased in ISO-induced hypertrophic rats; as compared with the model group, D. candidumcan inhibit ISO-induced ventricular pressure and ventricular hypertrophy, reduce myocardial collagen synthesis, improve myocardial fibrosis and ventricular remodeling, and significantly down-regulate ANP, BNP and cTN-I levels in plasma. This study shows that D. candidum has a protective effect on isoproterenol-induced cardiac hypertrophy.
