Viability of Refrigerated Human Articular Cartilage.
- Author:
Chang Whan HAN
1
;
Weon Yoo KIM
;
Jong Hun JEE
;
Dong Won CHOI
;
Jae Do HA
;
Freddie H FU
Author Information
1. Department of Orthopaedic Surgery, Daejeon St. Mary's hospital, College of Medicine, the Catholic University, Korea. chang911@djsungmo.com
- Publication Type:Original Article
- Keywords:
Articular cartilage allograft;
Chondrocyte;
Refrigeration;
Chondrocyte viability;
Proteoglycan synthesis
- MeSH:
Ankle;
Cartilage, Articular*;
Chondrocytes;
Humans*;
Proteoglycans;
Refrigeration;
Talus
- From:Journal of Korean Orthopaedic Research Society
2002;5(2):118-123
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To determine the viability of human chondrocytes within refrigerated articular cartilage stored under conditions currently used clinically. MATERIALS AND METHODS: Osteochondral sections of human ankle taluses were stored at 4 degrees C in DMEM media for 1 to 42 days. Articular cartilage was harvested and evaluated for histologic changes and proteoglycan synthesis. RESULTS: By day 7, markedly decreased proteoglycan synthesis was observed. After 21 days, synthetic activity was virtually undetectable. Histologic specimens demonstrated chondrocyte death of a half of the cells from the superficial layer at day 7. Within 21 days, significant chondrocyte death was seen. CONCLUSION: These results suggest that proteoglycan synthetic activity and chondrocyte viability are markedly decreased in articular cartilage after cold storage for longer than 7 days.
