Effect of exogenous GA₃ and DPC on active components of Lonicera japonica and its mechanism.
10.19540/j.cnki.cjcmm.20181106.007
- Author:
Yan-Xia ZHU
1
;
Shuang-Shuang QIN
1
;
Chao JIANG
2
;
Jian YANG
2
;
Qing-Hua WU
1
;
Ying LIANG
1
Author Information
1. Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement, Guangxi Botanical Garden of Medicinal Plant, Nanning 530023, China.
2. National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
- Publication Type:Journal Article
- Keywords:
Lonicera japonica;
chlorogenic acid;
endogenous hormones;
luteolin
- MeSH:
Chlorogenic Acid;
Lonicera;
Plant Growth Regulators;
Tandem Mass Spectrometry
- From:
China Journal of Chinese Materia Medica
2018;43(24):4817-4823
- CountryChina
- Language:Chinese
-
Abstract:
To reveal the effect of plant growth regulator GA₃ and DPC on the active components and its possible mechanism of Lonicera japonica. GA and DPC were applied at the stage of flower bud differentiation, and the content of active ingredients was measured by LC-MS-MS, the content of endogenous hormones were measured by ELISA, and the expression of key enzyme enes expression was determined by qRT-PCR. The level of endogenous hormone GA₃, IAA, ZR, DHZR and iPA in the GA treatment group was significantly improved, the expression of C4H1, C4H2, 4CL1 and HQT2 were also significantly increased. The content of chlorogenic acid, luteolin, luteoloside, isoquercetin and caffeic acid increased significantly. Spraying DPC did not affect or inhibit the accumulation of active components of L. japonica. Spraying GA can increase the content of endogenous GA₃, thus enhance the expression of C4H1, C4H2, 4CL1 and HQT2, and then increase the content of chlorogenic acid and luteolin.
