Zoological origin survey of commercial hippocampus in Chinese herbal markets by morphological and DNA sequencing identification.
10.19540/j.cnki.cjcmm.20181025.011
- Author:
Chao JIANG
1
;
Fu-Yan LIU
1
;
Yan JIN
1
;
Yuan YUAN
1
;
Yu-Yang ZHAO
1
;
Lu-Qi HUANG
1
Author Information
1. State Key Laboratory of Dao-di Herbs Breeding Base, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
- Publication Type:Journal Article
- Keywords:
hippocampus;
identification;
market survey;
molecular
- MeSH:
Animals;
China;
Europe;
Sequence Analysis, DNA;
Smegmamorpha;
Surveys and Questionnaires
- From:
China Journal of Chinese Materia Medica
2018;43(23):4553-4561
- CountryChina
- Language:Chinese
-
Abstract:
Hippocampus is a precious animal medicine in Chinese herbal medicines. Numerous seahorse species possessing similar morphology were used as commercial hippocampus in herbal markets. Clarifing the zoological of commercial hippocampus in herbal markets is a crucial issue, which contributed to establish authentication and quality control standard. This study investigated 1 156 dried seahorse samples collected from eight main herbal markets using CO Ⅰ fragment DNA sequencing coupling with morphological identification. The results showed that 23 seahorse species were present in the China TCM market. Among them, five species were officially listed in China Pharmacopoeia, seven species namely winged seahorse (Hippocampus alatus), giraffe seahorse (H. camelopardalis), knysna seahorse (H. capensis), beibuwan seahorse (H. casscsio), half-spiny seahorse (H. semispinosus), Europe seahorse (H. hippocampus), zebra seahorse (H. zebra) were found in herbal markets for the first time. The present DNA sequences analysis coupling with morphological identification method could also use to survey the species origin of other Chinese herbal medicines in herbal markets.
