Multiplex PCR to simultaneous identification of five traditional Chinese medicinal seahorses.
10.19540/j.cnki.cjcmm.20181025.012
- Author:
Fu-Yan LIU
1
;
Yuan YUAN
1
;
Yan JIN
2
;
Wen QIN
1
;
Chao JIANG
1
;
Yu-Yang ZHAO
1
;
Lu-Qi HUANG
1
Author Information
1. State Key Laboratory of Dao-di Herbs Breeding Base, National Resource Center for Chinese Materia, China Academy of Chinese Medical Sciences, Beijing 100700, China.
2. Department of Biomedical, Beijing City University, Beijing 100083, China.
- Publication Type:Journal Article
- Keywords:
COⅠ sequence;
molecular identification;
multiplex PCR;
seahorse;
traditional Chinese medicine
- MeSH:
Animals;
DNA Primers;
DNA, Mitochondrial;
Multiplex Polymerase Chain Reaction;
Smegmamorpha
- From:
China Journal of Chinese Materia Medica
2018;43(23):4562-4568
- CountryChina
- Language:Chinese
-
Abstract:
Seahorse is one the most commonly used medicinal animal in China. Five species of Hippocampus are recorded as seahorse in the Chinese Pharmacopoeia. Because of the rapid decrease, several other Hippocampus species are often adulterants as medicinal seahorse in the herbal market, which compromise clinical efficacy and pose threat to endangered seahorse species conversation. Herein, a multiplex polymerase chain reaction (mPCR) method was developed to identify the biological sources of medicinal seahorses.Based on the sequences of mitochondrial DNA, five specific primers for Hippocampus trimaculatus, H. kelloggi, H. kuda, H. histrix and H. mohnikei (H. japonicus)were designed, respectively. Multiplex PCR yields the products of 155, 222, 292, 352, 458 bp amplicons in the present of DNA templates of H. kuda, H. mohnikei, H. kelloggi, H. histrix and H. trimaculatus, respectively. This multiplex PCR method which electrophoresis migration of different lengths of DNA bands allowed simultaneous identification of all the five medicinal seahorses in a single assay. It showed that this multiplex PCR assay is useful for the simultaneous identification the biological sources of complex multi-source samples, which could provide a useful tool for the quality control of seahorses.
