A real-time fluorescence quantitative PCR method for source identification of Crocus sativus and Carthamus tinctorius.
10.19540/j.cnki.cjcmm.20181025.005
- Author:
Quan-Fang ZHANG
1
;
Yan-Yan LIU
1
;
Qing-Qing TAN
1
;
Xin-Xin JIANG
1
;
Xue-Yan CHEN
1
;
Fu-Cun GE
2
;
Xun BU
1
;
Yong-Qing ZHANG
3
Author Information
1. Biotechnology Research Center, Shandong Academy of Agricultural Sciences/Laboratory of Applied Life Science, Shandong Academy of Agricultural Sciences, Ji'nan 250100, China.
2. Shandong Baiweitang Chinese Medicine Pieces Co., Ltd., Ji'nan 250108, China.
3. Institute of Natural Medicine, Shandong University of Traditional Chinese Medicine, Ji'nan 250355, China.
- Publication Type:Journal Article
- Keywords:
Carthamus tinctorius;
Crocus sativus;
SYBRGreen real-time fluorescence PCR;
source identification
- MeSH:
Carthamus tinctorius;
Crocus;
Real-Time Polymerase Chain Reaction
- From:
China Journal of Chinese Materia Medica
2018;43(23):4575-4581
- CountryChina
- Language:Chinese
-
Abstract:
The specific PCR primer was designed base on ITS2 sequence in GenBank, and we developed a SYBRGreen real-time fluorescence quantitative PCR system for identification of Crocus sativus and Carthamus tinctorius source. Compared with Chinese herbal medicine DNA barcode technique, this method showed characteristics of shorter time, higher specificity and sensitivity. Using this method to detect 15 samples, 4 were C. sativus, 8 were C. tinctorius, and the other 3 samples were none of them. The result was in accordance with Chinese herbal medicine DNA barcode. This study lays the foundation for identification of related Chinese medical materials.
