Screening, identification and activity evaluation of pancreatic lipase inhibition in Prunella vulgaris.
10.19540/j.cnki.cjcmm.2018.0123
- Author:
Ting-Gui CHEN
1
;
Li-Ya LI
1
;
Yu-Ru WEI
1
;
Li-Wei ZHANG
1
Author Information
1. Institute of Molecular Science, Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan 030006, China.
- Publication Type:Journal Article
- Keywords:
LC-MS/MS;
Prunella vulgaris;
immobilized enzyme;
pancreatic lipase inhibitor
- MeSH:
Chromatography, Liquid;
Lipase;
Plant Extracts;
Prunella;
Tandem Mass Spectrometry
- From:
China Journal of Chinese Materia Medica
2018;43(23):4665-4671
- CountryChina
- Language:Chinese
-
Abstract:
Pancreatic lipase (PL) inhibitors were firstly screened from Prunella vulgaris with PL immobilized on carboxylic acid-terminated magnetic nanoparticles, then these possible inhibitors were identified by LC-MS/MS and mixed standards. Finally, their inhibitory effects and types on PL were tested by p-nitrophenol method. The results showed that four PL inhibitors were screened out from P. vulgaris and confirmed by LC-MS/MS and mixed standards. The IC₅₈ and inhibition types were as follows: caffeic acid [(252.3±3.6) mg·L⁻¹, anti-competitive inhibition], rutin [(91.2±1.6)mg·L⁻¹, competitive inhibition], hesperidin [(31.5±4.4) mg·L⁻¹, competitive inhibition] and ursolic acid [(41.3±2.2) mg·L⁻¹, competitive inhibition]. Their inhibitive types and abilities on PL were related to their molecular size, hydrophobicity and the number of hydrogen bond with PL triplet.
