Application of DNA metabarcoding in species identification of Chinese herbal medicines.
10.19540/j.cnki.cjcmm.2019.0001
- Author:
Zhi-Xiang LIU
1
;
Jiang XU
1
;
Wei SUN
1
;
Yu-Hua SHI
1
;
Shi-Lin CHEN
1
Author Information
1. Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine,Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences Beijing 100700,China.
- Publication Type:Journal Article
- Keywords:
DNA metabarcoding;
mixed Chinese herbal medicines;
species identification
- MeSH:
Biodiversity;
DNA Barcoding, Taxonomic;
DNA, Plant;
genetics;
Drugs, Chinese Herbal;
analysis;
High-Throughput Nucleotide Sequencing;
Plants, Medicinal;
classification
- From:
China Journal of Chinese Materia Medica
2019;44(1):1-8
- CountryChina
- Language:Chinese
-
Abstract:
DNA metabarcoding,one rapid and robust method using specific standard DNA fragments,has been widely used for rapid species identification of a bulk sample through high-throughput sequencing technologies.While it has been widely used in the studies of metagenomics,animal and plant biodiversity,it has gradually come to be used as a profitable method in species identification of mixed Chinese herbal medicines.In this paper,we mainly summarize the current studies of the application of DNA metabarcoding in species identification of mixed Chinese herbal medicines.Moreover,high-throughput sequencing technologies adopted in those studies,such as Sanger,the next-generation,and third-generation sequencing technologies,are discussed.It is conducted to provide a theoretical guidance for the application of DNA metabarcoding in species identification of mixed Chinese herbal medicines and in more other biodiversity studies.
