Mechanism of β-carboline alkaloids inhibiting migration and invasion of SGC-7901 cells.
10.19540/j.cnki.cjcmm.20180914.001
- Author:
Tao XI
1
;
Huan XIA
2
;
Yu-Xiang FAN
3
;
Yong-Cheng CAO
4
;
Hong-Liang ZHANG
3
Author Information
1. Xinjiang Medical University Urumqi 830000,China.
2. Science of Nuclear Medicine,Cancer Hospital Affiliated to Xinjiang Medical University Urumqi 830011,China.
3. Tumor Division Ⅱ,Fourth Affiliated Hospital of Xinjiang Medical University Urumqi 830000,China.
4. Xinjiang Changji State Traditional Chinese Medicine Hospital Changji 831100,China.
- Publication Type:Journal Article
- Keywords:
SGC-7901 cells;
focal adhesion kinase;
β-carboline alkaloids
- MeSH:
Alkaloids;
pharmacology;
Carbolines;
pharmacology;
Cell Line, Tumor;
Cell Movement;
drug effects;
Cell Proliferation;
Focal Adhesion Kinase 1;
genetics;
Gene Expression Regulation, Neoplastic;
Gene Silencing;
Humans;
Neoplasm Invasiveness;
Stomach Neoplasms;
drug therapy;
pathology
- From:
China Journal of Chinese Materia Medica
2019;44(1):119-124
- CountryChina
- Language:Chinese
-
Abstract:
To explore the mechanism of β-carboline alkaloids inhibiting the migration and invasion of SGC-7901 cells and its correlation with FAK gene expression,CCK-8 method was used to determine the inhibitory rate of β-carboline alkaloids on the proliferation of gastric cancer SGC-7901 cells under different concentrations.The effect of β-carboline alkaloids on the migration and invasion of SGC-7901 cells was used by Transwell compartment.Detection of mRNA and protein expression of FAK genes were used by qRT-PCR and Western blot.Then si-FAK-1051 recombinant plasmid was transfected into SGC-7901 cells.FAK gene silencing effect was identified by qRT-PCR and Western blot technique again.Finally,the effects of FAK gene silencing on proliferation and migration of gastric cancer SGC-7901 cells were detected by CCK-8 kit and Transwell chamber assay respectively.With the increase of the concentration ofβ-carboline alkaloids,the inhibitory rate of SGC-7901 cells in human gastric cancer cells increased gradually,with IC5013.364 mg·L-1.The number of SGC-7901 cells of Transwell compartment in the positive experimental group(5-FU,5 mg·L-1) and the β-carboline alkaloids group decreased significantly(P<0.01) and the number of SGC-7901 cells in the β-carboline alkaloids group was significantly lower than that in the positive experimental group(P<0.01).Compared with the blank control group,the mRNA and protein expression level of FAK genes in the positive experimental group was significantly lower than that in the experimental group of β-carboline alkaloids(P<0.05).After transfection of si-FAK-1051 into gastric cancer SGC-7901 cells,the expression of mRNA and protein of FAK gene was significantly down regulated(P<0.05).SGC-7901 cell proliferation and cell migration ability also decreased significantly(P<0.05).β-carboline alkaloids are more effective than 5-FU in inhibiting migration and invasion of gastric cancer SGC-7901 cells,and the mechanism may be related to the inhibition of mRNA and protein expression of FAK gene by β-carboline alkaloids.
