Inhibitory effect of extract of Coptidis Rhizoma on invasion of Candida albicans hyphae in vitro.
10.19540/j.cnki.cjcmm.20180912.004
- Author:
Hui-Xia NIU
1
;
Qiang-Jun DUAN
1
;
Gao-Xiang SHI
1
;
Da-Qiang WU
1
;
Jing SHAO
1
;
Tian-Ming WANG
1
;
Chang-Zhong WANG
2
Author Information
1. Institute of Integrated Traditional Chinese and Western Medicine,Anhui University of Traditional Chinese Medicine Hefei 230012,China Institute of Integrated Traditional Chinese and Western Medicine,Anhui Academy of Traditional Chinese Medicine Hefei 230012,China.
2. Institute of Integrated Traditional Chinese and Western Medicine,Anhui University of Traditional Chinese Medicine Hefei 230012,China Institute of Integrated Traditional Chinese and Western Medicine,Anhui Academy of Traditional Chinese Medicine Hefei 230012,China Key Laboratory of Chinese Medicinal Formula of Anhui province Hefei 230012,China.
- Publication Type:Journal Article
- Keywords:
Candida albicans;
extract of Coptidis Rhizoma(ECR);
hyphae;
invasin
- MeSH:
Adenosine Triphosphatases;
genetics;
Candida albicans;
drug effects;
Drugs, Chinese Herbal;
pharmacology;
Fungal Proteins;
genetics;
Gene Expression Regulation, Fungal;
HSP70 Heat-Shock Proteins;
genetics;
Hyphae;
drug effects;
ultrastructure;
Microscopy, Electron, Scanning
- From:
China Journal of Chinese Materia Medica
2019;44(1):125-130
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this paper was to investigate the inhibitory effect of extract of Coptidis Rhizoma(ECR) on invasion of Candida albicans hyphae in vitro.XTT reduction method was used to evaluate the metabolic activity of C.albicans.The colony edge growth of C.albicans was observed by solid medium.The growth of C.albicans hyphae was determined on semi-solid medium.The morphology and viability changes of C.albicans hyphae were assessed by scanning electron microscope and fluorescence microscope.qRT-PCR method was used to detect the ALS3 and SSA1 expression of C.albicans invasin genes.The results showed that the metabolic viability by XTT method detected that the activity of C.albicans was gradually decreased under the intervention of 64,128 and 256 mg·L-1 of ECR respectively.128,256 mg·L-1 of ECR significantly inhibited colony folds and wrinkles on solid medium and the hyphal invasion in semi-solid medium.Scanning electron microscopy and fluorescence microscopy showed that 128,256 mg·L-1 of ECR could inhibit the formation of C.albicans hyphae.qRT-PCR results showed that the expression of invasin gene ALS3 and SSA1 was down-regulated,and especially 256 mg·L-1 of ECR could down-regulate the two genes expression by 4.8,1.68 times respectively.This study showed that ECR can affect the invasiveness of C.albicans by inhibiting the growth of hyphae and the expression of invasin.
