ADAM17 knockdown increases sensitivity of SW480 cells to cetuximad.
10.12122/j.issn.1673-4254.2018.11.15
- Author:
Ying CHEN
1
;
Kehong ZHENG
1
;
Zetao CHEN
1
;
Haizhan FENG
1
;
Wei FANG
1
;
Zonghai HUANG
1
Author Information
1. Department of General Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510280, China.
- Publication Type:Journal Article
- Keywords:
ADAM17;
cetuximad;
drug resistance;
human colorectal cancer
- MeSH:
ADAM17 Protein;
genetics;
metabolism;
Antineoplastic Agents, Immunological;
pharmacology;
Apoptosis;
Cell Line, Tumor;
Cell Movement;
Cell Proliferation;
Cetuximab;
pharmacology;
Colorectal Neoplasms;
drug therapy;
genetics;
metabolism;
pathology;
Drug Resistance, Neoplasm;
genetics;
ErbB Receptors;
metabolism;
Gene Knockdown Techniques;
Humans;
Neoplasm Invasiveness;
Oncogene Protein v-akt;
metabolism;
RNA, Small Interfering;
Signal Transduction;
Transfection;
methods
- From:
Journal of Southern Medical University
2018;38(11):1366-1371
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the association between expression of ADAM17 and cetuximad resistance in human colorectal cancer SW480 cells.
METHODS:The expression of ADAM17 was detected using Western blotting in different human colorectal cancer cell lines, and the cells highly expressing ADAM17 were selected as the target cells. SW480 cells were transfected with ADAM17-siRNA 1 and ADAM17-siRNA 2 and the changes in the expression of ADAM17 protein were detected using Western blotting. SW480 cells were exposed to cetuximad for 24 h and the cell apoptosis was analyzed using flow cytometry. Transwell assay was used to examine the migration ability of SW480 cells with different expression levels of ADAM17; Western blotting was used to analyze the changes in the expressions of AKT signaling pathway-related proteins in the treated cells.
RESULTS:The baseline expressions of ADAM17 were significantly higher in SW480 cells than in the other human colorectal cancer cell lines tested ( < 0.05). Both ADAM17-siRNA 1 and 2 effectively reduced the expression of ADAM17 protein in SW480 cells. Knockdown of ADAM17 with siRNA 1 significantly increased the sensitivity of SW480 cells to tocetuximad ( < 0.05), obviously inhibited the cell proliferation, migration and invasion, and significantly reduced the expressions of p-EGFR and p-AKT in the cells ( < 0.001).
CONCLUSIONS:ADAM17 knockdown obviously inhibits EGFR-AKT signaling pathway and increases the sensitivity of SW480 cells to tocetuximad.
