Anti-scarring effect of rapamycin in rabbits following glaucoma filtering surgery.
10.12122/j.issn.1673-4254.2018.11.19
- Author:
Xin KANG
1
;
Ying SHEN
1
;
Haixia ZHAO
1
;
Zhaoge WANG
1
;
Wenying GUAN
1
;
Ruichun GE
1
;
Ruifang WANG
1
;
Xue TAI
1
Author Information
1. Center of Myopia, Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010050, China.
- Publication Type:Journal Article
- Keywords:
anti-scarring agents;
apoptosis;
filtration surgery;
glaucoma;
rapamycin
- MeSH:
Animals;
Caspase 3;
metabolism;
Caspase 9;
metabolism;
Cell Proliferation;
drug effects;
Cicatrix;
prevention & control;
Filtering Surgery;
adverse effects;
Glaucoma;
surgery;
Intraocular Pressure;
Postoperative Complications;
enzymology;
prevention & control;
Proliferating Cell Nuclear Antigen;
analysis;
Rabbits;
Random Allocation;
Sirolimus;
therapeutic use;
Trabeculectomy
- From:
Journal of Southern Medical University
2018;38(11):1389-1394
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the anti- scarring effect of rapamycin in rabbits receiving glaucoma filtering surgery.
METHODS:Ninety-six Chinchilla rabbits were randomized equally into 3 rapamycin treatment groups and one control group. All the rabbits underwent trabeculectomy, after which the rabbits in the 3 rapamycin groups were treated with eye drops containing 1%, 3%, or 5% rapamycin in the operated eyes, and those in the control groups were given castor oil 4 times a day. The intraocular pressure (IOP) and inflammatory reaction in the treated eyes were observed, and the PCNA-positive cells in the filtering bleb were detected using immunohistochemistry. RTFs isolated from the Tenon's capsule of the rabbits were cultured , and the expressions of caspase-3, caspase-8, and caspase-9 in the fibroblasts were detected after treatment with different concentrations of rapamycin.
RESULTS:The IOP was significantly lower in rapamycin-treated group than in the control group after the surgery ( < 0.05). The counts of the PCNA-positive cells were significantly lower in rapamycin-treated rabbits than in the control group ( < 0.05). Rapamycin treatment dose-dependently increased the expressions of caspase-3 and caspase- 9 at both the mRNA ( < 0.001) and protein ( < 0.001) levels without causing significant changes in the expressions of caspase-8.
CONCLUSIONS:Rapamycin can inhibit excessive proliferation of the fibroblasts in the filtering bleb to reduce scar formation after glaucoma filtration surgery in rabbits. Rapamycin also increases the expressions of caspase-3 and caspase-9 to induce apoptosis of the RTFs.
