Expression of miR-454-3p and its effect on proliferation, invasion and metastasis of colon cancer.
10.12122/j.issn.1673-4254.2018.12.04
- Author:
Wenhua LI
1
;
Yun FENG
1
;
Zhenzeng MA
1
;
Lungen LU
1
Author Information
1. Department of Gastroenterology, Shanghai General Hospital of Nanjing Medical University, Shanghai 201600, China.
- Publication Type:Journal Article
- Keywords:
colon cancer;
invasion;
metastasis;
microRNA
- MeSH:
Animals;
Biomarkers, Tumor;
metabolism;
Cell Line, Tumor;
Cell Movement;
Cell Proliferation;
Colon;
metabolism;
Colonic Neoplasms;
metabolism;
pathology;
Disease Progression;
Humans;
Liver Neoplasms;
secondary;
Mice;
Mice, Inbred BALB C;
MicroRNAs;
metabolism;
Neoplasm Invasiveness;
Sincalide;
metabolism
- From:
Journal of Southern Medical University
2018;38(12):1421-1426
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the expression of miR-454-3p in colon cancer and its effect on colon cancer proliferation, invasion and hepatic metastasis.
METHODS:Specimens of tumor tissues and paired adjacent tissues were collected from 20 patients with colorectal cancer for detecting the expression levels of miR-454-3p using in situ hybridization. Colon cancer cell line SW480 was transfected with a lentiviral vector to induce miR-454-3p over-expression, and the changes in cell proliferation and invasion were observed using cell counting kit-8 (CCK-8), clone formation assay and Transwell experiment. The effect of miR- 454-3p over-expression on hepatic metastasis of colon cancer was assessed in BALB/c mouse models.
RESULTS:The results of in situ hybridization showed a significantly increased expression level of miR-454-3p in colon cancer tissues as compared with normal colon tissues ( < 0.05). In SW480 cells, over-expression of miR-454-3p significantly promoted the cell proliferation and invasion ( < 0.05). In BALB/c mice, SW480 cells over-expressing miR-454-3p showed a significantly higher potential for liver metastases than the control cells ( < 0.05).
CONCLUSIONS:miR-454-3p is overexpressed in the tumor tissues in patients with colorectal cancer and participates in the progression of colorectal cancer by promoting cancer cell proliferation, invasion, and liver metastasis. miR-454-3p may serve as a novel biomarker for colorectal cancer diagnosis and prognostic evaluation.
