Effects of Polygonum cuspidatum on AMPK-FOXO3α Signaling Pathway in Rat Model of Uric Acid-Induced Renal Damage.
10.1007/s11655-017-2979-6
- Author:
Wei-Guo MA
1
;
Jie WANG
2
;
Xiang-Wei BU
1
;
Hong-Hong ZHANG
1
;
Jian-Ping ZHANG
1
;
Xiao-Xu ZHANG
1
;
Yu-Xi HE
1
;
Da-Li WANG
1
;
Zheng-Ju ZHANG
1
;
Feng-Xian MENG
3
Author Information
1. Department of Rheumatology, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing, 100078, China.
2. Department of Endocrinology, Shunyi Branch, Beijing Hospital of Traditional Chinese Medicine, Beijing, 101300, China.
3. Department of Rheumatology, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing, 100078, China. mfx0823@163.com.
- Publication Type:Journal Article
- Keywords:
AMPK-FOXO3α pathway;
Chinese medicine;
Polygonum cuspidatum;
renal damage;
uric acid
- MeSH:
AMP-Activated Protein Kinases;
physiology;
Animals;
Chemokine CCL2;
blood;
Disease Models, Animal;
Fallopia japonica;
Forkhead Box Protein O3;
physiology;
Hyperuricemia;
complications;
Kidney Diseases;
drug therapy;
etiology;
Male;
Plant Extracts;
pharmacology;
Rats;
Rats, Sprague-Dawley;
Signal Transduction;
drug effects;
Uric Acid
- From:
Chinese journal of integrative medicine
2019;25(3):182-189
- CountryChina
- Language:English
-
Abstract:
BACKGROUND:To observe the effects of Chinese medicine (CM) Polygonum cuspidatum (PC) on adenosine 5'-monophosphate-activated protein kinase (AMPK), forkhead box O3α (FOXO3α), Toll-like receptor-4 (TLR4), NACHT, LRR and PYD domains-containing protein 3 (NLRP3), and monocyte chemoattractant protein-1 (MCP-1) expression in a rat model of uric acid-induced renal damage and to determine the molecular mechanism.
METHODS:A rat model of uric acid-induced renal damage was established, and rats were randomly divided into a model group, a positive drug group, and high-, medium-, and low-dose PC groups (n=12 per group). A normal group (n=6) was used as the control. Rats in the normal and model groups were administered distilled water (10 mL•kg) by intragastric infusion. Rats in the positive drug group and the high-, medium-, and low-dose PC groups were administered allopurinol (23.33 mg•kg), and 7.46, 3.73, or 1.87 g•kg•d PC by intragastric infusion, respectively for 6 to 8 weeks. After the intervention, reverse transcription polymerase chain reaction, Western blot, enzyme linked immunosorbent assay, and immunohistochemistry were used to detect AMPK, FOXO3α, TLR4, NLRP3, and MCP-1 mRNA and protein levels in renal tissue or serum.
RESULTS:Compared with the normal group, the mRNA transcription levels of AMPK and FOXO3α in the model group were significantly down-regulated, and protein levels of AMPKα1, pAMPKα1 and FOXO3α were significantly down-regulated at the 6th and 8th weeks (P<0.01 or P<0.05). The mRNA transcription and protein levels of TLR4, NLRP3 and MCP-1 were significantly up-regulated (P<0.01 or P<0.05). Compared with the model group, at the 6th week, the mRNA transcription levels of AMPK in the high- and medium-dose groups, and protein expression levels of AMPKα1, pAMPKα1 and FOXO3α in the high-dose PC group, AMPKα1 and pAMPKα1 in the mediumdose PC group, and pAMPKα1 in the low-dose PC group were significantly up-regulated (P<0.01 or P<0.05); the mRNA transcription and protein levels of TLR4 and NLRP3 in the 3 CM groups, and protein expression levels of MCP-1 in the medium- and low-dose PC groups were down-regulated (P<0.01 or P<0.05). At the 8th week, the mRNA transcription levels of AMPK in the high-dose PC group and FOXO3α in the medium-dose PC group, and protein levels of AMPKα1, pAMPKα1 and FOXO3α in the 3 CM groups were significantly up-regulated (P<0.01 or P<0.05); the mRNA transcription levels of TLR4 in the medium- and low-dose PC groups, NLRP3 in the high- and low-dose PC groups and MCP-1 in the medium- and low-dose PC groups, and protein expression levels of TLR4, NLRP3 and MCP-1 in the 3 CM groups were down-regulated (P<0.01 or P<0.05).
CONCLUSION:PC up-regulated the expression of AMPK and its downstream molecule FOXO3α and inhibited the biological activity of TLR4, NLRP3, and MCP-1, key signal molecules in the immunoinflammatory network pathway, which may be the molecular mechanism of PC to improve hyperuricemia-mediated immunoinflflammatory metabolic renal damage.
