Shexiang Tongxin Dropping Pill () Protects against NaSO-Induced Hypoxia-Reoxygenation Injury in H9c2 Cells.
10.1007/s11655-018-2976-4
- Author:
Shan LIN
1
;
Jiu-Mao LIN
1
;
Ling ZHANG
1
;
Da-Xin CHEN
1
;
Fei XIAO
1
;
Hong-Wei CHEN
1
;
You-Qin CHEN
2
;
Yu-Ling ZHU
3
;
Jian-Feng CHU
1
;
Jun PENG
4
Author Information
1. Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, 350122, China.
2. Inner Mongolia Conba Pharmaceutical Co., Ltd., Shanghai, 201318, China.
3. CHEN Ke-ji Academic Thought Heritage Studio, Fuzhou, 350122, China.
4. Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, 350122, China. pjunlab@hotmail.com.
- Publication Type:Journal Article
- Keywords:
Chinese medicine;
Na2S2O4-induced hypoxia-reoxygenation injury;
Shexiang Tongxin Dropping Pill;
apoptosis;
myocardial ischemia
- From:
Chinese journal of integrative medicine
2019;25(6):439-445
- CountryChina
- Language:English
-
Abstract:
OBJECTIVES:To investigate the protective effects of Shexiang Tongxin Dropping Pill (, STP) on NaSO-induced hypoxia-reoxygenation injury in cardiomyoblast H9c2 cells.
METHODS:The cell viability and levels of mRNA and protein expression in H9c2 cells were determined following NaSO-induced hypoxia using Hoechst staining, annexin V/propidium iodide (PI) flow cytometry, real-time polymerase chain reaction and Western blot analysis.
RESULTS:STP pretreatment significantly increased the viability and inhibited aberrant morphological changes in H9c2 cardiomyoblast cells induced by NaSO treatment (P<0.05). In addition, STP pretreatment attenuated NaSO-induced hypoxic damage, down-regulated the expression of pro-apoptotic Bax, and up-regulated the expression of anti-apoptotic Bcl-2 in H9c2 cells (P<0.05).
CONCLUSIONS:STP was strongly cardioprotective in hypoxia-reoxygenation injury by preventing hypoxic damage and inhibiting cellular apoptosis. These results further support the use of STP as an effective drug for the treatment of ischemic heart disease.
