Model for fitness burden imposed by exogenous gene expression in quorum sensing bacteria.

Author: Fang LUO ¹ ; Yi YU ¹ ; Mingzhe CHEN ¹ ; Yiqing YANG ¹ ; Yin WEI ¹
Author Information

1. School of Life Science and Technology, Shanghai Tech University, Shanghai 201210, China.
Publication Type:Journal Article
Keywords: bacterial growth; fitness burden; gene expression; quorum sensing; transcription factor
MeSH: Bacteria; Gene Expression; Gene Expression Regulation, Bacterial; Quorum Sensing
From: Chinese Journal of Biotechnology 2018;34(12):1895-1905
CountryChina
Language:Chinese
Abstract: The exogenous gene expression and its impacts on the bacterial population are important to study quorum sensing systems and synthetic biology industry. However, the behavior of exogenous protein expressing bacteria remains poorly understood. To find out which factors are playing a critical role in the growth of population and exogenous gene expression, we measured Lux-type receptor-regulated exogenous gene expression under the induction of N-acyl homoserine lactone (N-AHL) signaling molecules and impacts on the bacterial population dynamics after such stimulation. To analyze the cause of fitness burden of bacteria, we set up a hypothetical mathematical model. Previous studies often arrogate this phenomenon to the synthesis cost and the toxicity of N-AHL signaling molecule. However, we suggested another possible cause of the fitness burden.