Methods for mammalian single cell research - a review.

Author: Wenqian JIANG ¹ ; Yarong TIAN ² ; Rui ZUO ¹ ; Jun LIN ¹
Author Information

1. Institute of Applied Genomics, Fuzhou University, Fuzhou 350108, Fujian, China.
2. Department of Infectious Diseases, Institute of Biomedicine, University of Gothenburg, Gothenburg 40530, Sweden.
Publication Type:Journal Article
Keywords: laser capture microdissection (LCM); microfluidic; single cell; single cell genome sequencing; single cell transcriptome sequencing
MeSH: Animals; Cell Separation; DNA; Laser Capture Microdissection; RNA; Single-Cell Analysis
From: Chinese Journal of Biotechnology 2019;35(1):27-39
CountryChina
Language:Chinese
Abstract: Basic research in life science and medicine has dug into single cell level in recent years. Single-cell analysis offers to understand life from diverse perspectives and is used to profile cell heterogeneity to investigate mechanism of diseases. Single cell technologies have also found applications in forensic medicine and clinical reproductive medicine, while the techniques are rapidly evolving and have become more and more sophisticated. In this article, we reviewed various single cell isolation techniques and their pros and cons, including manual cell picking, laser capture microdissection and microfluidics, as well as analysis methods for DNA, RNA and protein in single cell. In addition, we summarized major up-to-date single cell research achievements and their potential applications.