Advances in antibody drug expression techniques.
- Author:
Mengxiao ZHANG
1
;
Jianwei ZHU
1
;
Huili LU
1
Author Information
- Publication Type:Journal Article
- Keywords: CRISPR; antibody drugs; expression system; mammalian cells; manufacturing
- MeSH: Animals; Antibodies, Monoclonal; Biotechnology; Cell Line; Escherichia coli; Hybridomas
- From: Chinese Journal of Biotechnology 2019;35(2):171-182
- CountryChina
- Language:Chinese
- Abstract: The 21st century is regarded as the century of biotechnological drugs, among which monoclonal antibodies and their derived targeting drugs have established themselves as the leading modality of biopharmaceutical pharmaceutics for a wide range of indications covering malignant tumors and autoimmune disorders. Since the manufacturing of the first antibody drug from hybridoma cells, the technologies have been intensely studied and there emerged numerous breakthroughs in recombinant cell line establishment, antibody expression and purification, quality control and other related areas. This article summarizes the critical progresses of antibody drugs expression technologies, especially of mammalian cell expression system, Escherichia coli expression system, the transgenic animal reactor and the cell free protein synthesis system, to give a detailed illustration of the recent advances in antibody drugs development.
