Microbial production of poly (glycolate-co-lactate-co-3-hydroxybutyrate) from glucose and xylose by Escherichia coli.
- Author:
Yangyang DA
1
;
Wei LI
1
;
Lilong SHI
1
;
Zhengjun LI
1
Author Information
- Publication Type:Journal Article
- Keywords: 3-hydroxybutyrate; glucose; glycolate; lactate; polyhydroxyalkanoate; xylose
- MeSH: 3-Hydroxybutyric Acid; Escherichia coli; Glucose; Glycolates; Lactates; Metabolic Engineering; Polyesters; Xylose
- From: Chinese Journal of Biotechnology 2019;35(2):254-262
- CountryChina
- Language:Chinese
- Abstract: Escherichia coli was metabolically engineered to produce poly(glycolate-co-lactate-co-3-hydroxybutyrate) using glucose and xylose as carbon sources. The combinatorial biosynthetic route was constructed by the overexpression of a series of enzymes including D-tagatose 3-epimerase, L-fuculokinase, L-fuculose-phosphate aldolase, aldehyde dehydrogenase, propionyl-CoA transferase, β-ketothiolase, acetoacetyl-CoA reductase, and polyhydroxyalkanoate synthase. Overexpression of polyhydroxyalkanoate granule associated protein significantly improved biopolymer synthesis, and the recombinant strain reached 3.73 g/L cell dry weight with 38.72% (W/W) biopolymer content. A co-culture engineering strategy was developed to produce biopolymer from a mixture of glucose and xylose, achieving 4.01 g/L cell dry weight containing 21.54% (W/W) biopolymer. The results of this work offer an approach for simultaneously utilizing glucose and xylose and indicate the potential for future biopolymer production from lignocellulosic biomass.
