Analysis of genomic DNA methylation level in foxtail millet by Methylation Sensitive Amplified Polymorphism.

Yuxin Zhang; Yanan Ruan; Chen Zhao; Minmin Xue; Bo LI; Jingjing Wang; Yang Liu; Kaixi Wang; Hongyan Wang

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Analysis of genomic DNA methylation level in foxtail millet by Methylation Sensitive Amplified Polymorphism.

Author: Yuxin ZHANG ¹ ; Yanan RUAN ¹ ; Chen ZHAO ¹ ; Minmin XUE ¹ ; Bo LI ¹ ; Jingjing WANG ¹ ; Yang LIU ¹ ; Kaixi WANG ² ; Hongyan WANG ¹
Author Information

1. Laboratory of Plant Epigenetics and Evolution, School of Life Sciences, Liaoning University, Shenyang 110036, Liaoning, China.
2. Liaoning Institute of Soil and Water Conservation, Chaoyang 122000, Liaoning, China.
Publication Type:Journal Article
Keywords: DNA methylation; foxtail millet (Setaria italica); methylation-sensitive amplification polymorphism (MSAP)
MeSH: DNA Methylation; Genome, Plant; Genomics; Polymorphism, Genetic; Setaria Plant
From: Chinese Journal of Biotechnology 2019;35(2):263-269
CountryChina
Language:Chinese
Abstract: DNA methylation is an important type of epigenetic modification in eukaryotes. In order to research genome-wide methylation levels and patterns in foxtail millet (Setaria italica), the Methylation Sensitive Amplified Polymorphism (MSAP) analysis (employing double digestion with EcoR I and Hpa II/Msp I) was established and applied in two foxtail millet cultivars (Chaogu 58 and Yugu 1). The results showed that 32 pairs of MSAP primers were selected from 100 MSAP primers, and 1 615 and 1 482 clearly distinguishable and reproducible bands were amplified from Chaogu 58 and Yugu 1 respectively, including 3 types of methylation patterns. Cytosine methylation levels of CCGG context in Chaogu 58 and Yugu 1 were characterized as 6.93% and 8.77% respectively. Such different genomic DNA methylation levels between two foxtail millet varieties may provide a preliminary reference for the cultivation of this crop from a novel epigenetic viewpoint.