Application and optimization of CRISPR/Cas system in bacteria.

Author: Junhao FU ¹ ; Fayu YANG ¹ ; Haihua XIE ¹ ; Feng GU ¹
Author Information

1. State Key Laboratory of Ophthalmology and Optometry, School of Ophthalmology and Optometry, Wenzhou Medical University, Wenzhou 325027, Zhejiang, China.
Publication Type:Journal Article
Keywords: CRISPR/Cas system; bacteria; genome editing; optimization
MeSH: Animals; Bacteria; CRISPR-Cas Systems; Endonucleases; Gene Editing; Humans; Mice
From: Chinese Journal of Biotechnology 2019;35(3):341-350
CountryChina
Language:Chinese
Abstract: Clustered regular interspaced short palindromic repeats (CRISPR) system has been widely used in recent years. Compared with traditional genome editing technology, CRISPR/Cas system has notable advantages, including high editing efficiency, high specificity, low cost and the convenience for manipulation. Type Ⅱ and Ⅴ CRISPR/Cas system only requires a single Cas9 protein or a single Cpf1 protein as effector nucleases for cutting double-stranded DNA, developed as genome editing tools. At present, CRISPR/Cas9 technology has been successfully applied to the genome editing of eukaryotes such as zebrafish, mice and human cells, whereas limited progress has been made in the genome editing of bacteria. In our review, we describe CRISPR/Cas system, its mechanism and summarize the optimization and progress of genome editing in bacteria.