Construction and application of mCherry red fluorescent protein fusion expression system in lactic acid bacteria.
- Author:
Ying CHEN
1
;
Peijuan WANG
1
;
Wenjun ZHANG
1
;
Qiuxu YANG
1
;
Yao YANG
1
Author Information
- Publication Type:Journal Article
- Keywords: Lactobacillus plantarum; fusion expression; mCherry red fluorescent protein; pSIP vector; promoter; salt hydrolase
- MeSH: Lactobacillus plantarum; Luminescent Proteins; Probiotics
- From: Chinese Journal of Biotechnology 2019;35(3):492-504
- CountryChina
- Language:Chinese
- Abstract: In order to enrich the library of domestic research about new red fluorescent marker in lactic acid bacteria (LAB), we described a new fusion expression system in Lactobacillus plantarum WCFS1 based on the pSIP vector. This system contained red fluorescent protein mCherry as a marker and bile salt hydrolase gene (bsh) as a reporter gene. Moreover, in this study, four different promoters (PsppA, PldhL, P32 and PslpA) were used to regulate the expression of the fusion protein mCherry-BSH, completing the inducible and constitutive expression in lactic acid bacteria. The recombinant protein mCherry-BSH presented activity of red fluorescence and bile salt hydrolase (BSH). The successful construction of the fusion expression system in LAB using a red fluorescent protein mCherry provides favorable conditions for the distribution, intestinal colonization and survival rate of lactic acid bacteria, so as to reveal the function mechanism of its probiotic characteristics; and the system also could lay the foundation for researches on protein expression, cellular localization and properties identification of active protein in lactic acid bacteria.
