Advances in isopeptide bond-mediated molecular superglue.

Author: Deying GAO ¹ ; Jiawen GAO ¹ ; Xiaobao SUN ¹ ; Kexin ZHOU ¹ ; Tietao ZHANG ² ; Qian WANG ¹
Author Information

1. College of Biological & Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, Zhejiang, China.
2. Institute of Special Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences, Changchun 130112, Jilin, China.
Publication Type:Journal Article
Keywords: SpyTag/SpyCatcher; application; isopeptide bond; mechanism; molecular cyclization
MeSH: Cyclization; Lysine; Peptides; chemistry; Proteins
From: Chinese Journal of Biotechnology 2019;35(4):607-615
CountryChina
Language:Chinese
Abstract: Isopeptide bond-mediated molecular superglue is the irreversible covalent bond spontaneously formed by the side chains of lysine (Lys) and asparagine/aspartic acid (Asn/Asp) residues. The peptide-peptide interaction is specific, stable, and can be achieved quickly without any particular physicochemical factor. In the light of recent progress by domestic and foreign researchers, here we summarize the origin, assembly system and mechanism of isopeptide bond reaction, as well as the molecular cyclization and protein topological structure mediated by it. The prospect for its application in synthetic vaccine, hydrogel and bacterial nanobiological reactor is further discussed.