Progress in gene knockout mice.
- Author:
Chaoxuan WANG
1
;
Hang SUN
1
Author Information
- Publication Type:Journal Article
- Keywords: embryonic stem cells; gene editing; gene knockout technology; gene targeting; homologous recombination
- MeSH: Animals; Disease Models, Animal; Embryonic Stem Cells; Gene Knockout Techniques; trends; Gene Targeting; trends; Homologous Recombination; Humans; Mice; Mice, Knockout
- From: Chinese Journal of Biotechnology 2019;35(5):784-794
- CountryChina
- Language:Chinese
- Abstract: The establishment and development of gene knockout mice have provided powerful support for the study of gene function and the treatment of human diseases. Gene targeting and gene trap are two techniques for generating gene knockout mice from embryonic stem cells. Gene targeting replaces endogenous knockout gene by homologous recombination. There are two ways to knock out target genes: promoter trap and polyA trap. In recent years, many new gene knockout techniques have been developed, including Cre/loxP system, CRISP/Cas9 system, latest ZFN technology and TALEN technology. This article focuses on the several new knockout mouse techniques.
