Pyramiding of 3-resistant-gene to improve rice blast resistance of a restorer line, Fuhui 673.

Zhiwei Chen; Huazhong Guan; Xiaofang Wang; Ruixia Dong; Chenghai Zhuo; Damei Mao; Runsen Pan; Yuanchang Zhou; Weiren WU

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Pyramiding of 3-resistant-gene to improve rice blast resistance of a restorer line, Fuhui 673.

Author: Zhiwei CHEN ¹ ; Huazhong GUAN ¹ ; Xiaofang WANG ² ; Ruixia DONG ² ; Chenghai ZHUO ¹ ; Damei MAO ¹ ; Runsen PAN ¹ ; Yuanchang ZHOU ¹ ; Weiren WU ¹
Author Information

1. Fujian Provincial Key Laboratory of Crop Breeding by Design, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China.
2. Rice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350003, Fujian, China.
Publication Type:Journal Article
Keywords: backcross breeding; gene pyramiding; genetic improvement; marker-assisted selection; resistance to rice blast
MeSH: Breeding; Disease Resistance; genetics; Genes, Plant; genetics; Oryza; genetics
From: Chinese Journal of Biotechnology 2019;35(5):837-846
CountryChina
Language:Chinese
Abstract: To improve the blast resistance of elite rice restorer line Fuhui 673, 3 blast resistance genes Pi-1, Pi-9 and Pi-kh were introduced into Fuhui 673 from a good-quality restorer line Jinhui 1059 through 3 successive backcrosses followed by one selfing using the technique of marker-assisted selection. Ten near-isogenic lines (NILs) of Fuhui 673 carrying the 3 introduced resistance genes were created. Genotype analysis using 68 SSR markers evenly distributed in the genome indicated that 92.96%-98.59% of the NILs' genetic background had been recovered to Fuhui 673. Both indoor and field resistance tests indicated that the NILs and their hybrids with sterile line Yixiang A were all resistant to rice blast, with resistance levels significantly higher than those of controls Fuhui 673 and hybrid Yiyou 673 (Yixiang A  Fuhui 673). In addition, among the 10 hybrids between the NILs and Yixiang A, 2 showed significantly higher yield than and 4 displayed similar yield to that of control Yiyou 673, suggesting that most of the NILs retained the elite characteristics of Fuhui 673. Two new hybrid rice cultivars Liangyou 7283 and Jintaiyou 683 from NIL Line 9 showed high yield, good resistance to blast and moderate growth period in regional trial, suggesting that the NIL Line 9 has a good prospect for application.