Purification and bacteriostatic identification of CpxP protein from Pectobacterium carotovorum subsp. carotovorum.
- Author:
Lantian MIAO
1
;
Tianhua LU
1
;
Xiaoliang HE
1
;
Xiaohui ZHOU
1
Author Information
- Publication Type:Journal Article
- Keywords: CpxP; Pectobacterium carotovorum subsp. carotovorum; bacteriostasis; purification
- MeSH: Anti-Bacterial Agents; pharmacology; Bacteria; drug effects; Bacterial Proteins; isolation & purification; pharmacology; Escherichia coli; genetics; Membrane Proteins; isolation & purification; pharmacology; Pectobacterium carotovorum; genetics; metabolism; Plasmids; genetics
- From: Chinese Journal of Biotechnology 2019;35(5):847-856
- CountryChina
- Language:Chinese
- Abstract: Pectobacterium carotovorum subsp. carotovorum is one of the world's top ten plant pathogens, mainly infecting cruciferous economic crops and ornamental flowers. In this study, an antibacterial gene cpxP (Gene ID: 29704421) was cloned from the genome of Pectobacterium carotovorum subsp. carotovorum, and constructed on the prokaryotic expression plasmid pET-15b, and the recombinant plasmid was transformed into Escherichia coli BL21 (DE3), then stability and bacteriostatic experiments of the purified CpxP protein were performed. The final concentration of IPTG was 1 mmol/L, obtaining high-efficiency exogenous expression of the CpxP protein. There was no other protein after purification, and the destined protein exhibited good thermal stability and pH stability. The antibacterial test results showed that the inhibition rate of the CpxP protein on carrot slice was 44.89% while the inhibition rate on potato slice was 59.41%. To further explain its antibacterial mechanism, studying the spatial structure of this protein can provide new ideas for the control of soft rot and new protein pesticide targets.
