Growth suppression of colorectal cancer expressing S492R EGFR by monoclonal antibody CH12.
10.1007/s11684-019-0682-z
- Author:
Qiongna DONG
1
;
Bizhi SHI
1
;
Min ZHOU
1
;
Huiping GAO
1
;
Xiaoying LUO
1
;
Zonghai LI
1
;
Hua JIANG
2
Author Information
1. State Key Laboratory of Oncogenes & Related Genes, Shanghai Cancer Institute, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200032, China.
2. State Key Laboratory of Oncogenes & Related Genes, Shanghai Cancer Institute, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200032, China. jianghuapy@163.com.
- Publication Type:Journal Article
- Keywords:
S492R EGFR ectodomain mutation;
colorectal cancer;
immunnotherapy;
mAb CH12
- MeSH:
Animals;
Antibodies, Monoclonal;
pharmacology;
Antineoplastic Agents, Immunological;
pharmacology;
Caco-2 Cells;
Cell Proliferation;
drug effects;
Colorectal Neoplasms;
therapy;
ErbB Receptors;
genetics;
immunology;
Female;
HT29 Cells;
Humans;
Mice;
Mice, Inbred BALB C;
Mutation;
Xenograft Model Antitumor Assays
- From:
Frontiers of Medicine
2019;13(1):83-93
- CountryChina
- Language:English
-
Abstract:
Colorectal cancer (CRC) is a common malignant tumor in the digestive tract, and 30%-85% of CRCs express epidermal growth factor receptors (EGFRs). Recently, treatments using cetuximab, also named C225, an anti-EGFR monoclonal antibody, for CRC have been demonstrated to cause an S492R mutation in EGFR. However, little is known about the biological function of S492R EGFR. Therefore, we attempted to elucidate its biological function in CRC cells and explore new treatment strategies for this mutant form. Our study indicated that EGFR and S492R EGFR accelerate the growth of CRC cells in vitro and in vivo and monoclonal antibody CH12, which specifically recognizes an EGFR tumor-specific epitope, can bind efficiently to S492R EGFR. Furthermore, mAb CH12 showed significantly stronger growth suppression activities and induced a more potent antibody-dependent cellular cytotoxicity effect on CRC cells bearing S492R EGFR than mAb C225. mAb CH12 obviously suppressed the growth of CRC xenografts with S492R EGFR mutations in vivo. Thus, mAb CH12 may be a promising therapeutic agent in treating patients with CRC bearing an S492R EGFR mutation.
