Surface modification of PGP for a neutrophil-nanoparticle co-vehicle to enhance the anti-depressant effect of baicalein.

Baoyu Chen; Man Luo; Jianming Liang; Chun Zhang; Caifang Gao; Jue Wang; Jianxin Wang; Yongji LI; Desheng XU; Lina Liu; Ning Zhang; Huijun Chen; Jing Qin

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Surface modification of PGP for a neutrophil-nanoparticle co-vehicle to enhance the anti-depressant effect of baicalein.

Author: Baoyu CHEN ¹ ; Man LUO ² ; Jianming LIANG ^{3
,

4} ; Chun ZHANG ^{3
,

4} ; Caifang GAO ^{3
,

4} ; Jue WANG ^{3
,

4} ; Jianxin WANG ^{3
,

4} ; Yongji LI ² ; Desheng XU ¹ ; Lina LIU ^{3
,

4} ; Ning ZHANG ¹ ; Huijun CHEN ⁵ ; Jing QIN ^{3
,

4}
Author Information

1. Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
2. Department of Pharmaceutics, School of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin 150040, China.
3. Department of Pharmaceutics, School of Pharmacy, Fudan University
4. Key Laboratory of Smart Drug Delivery, Ministry of Education, Shanghai 201203, China.
5. The Second Hospital Affiliated Heilongjiang University of Traditional Chinese Medicine, Harbin 150001, China.
Publication Type:Journal Article
Keywords: Baicalein; Depression; Dual-brain targeting delivery; Neutrophils; Olfactory bulbectomy rats; PGP peptide; Solid lipid nanoparticle
From: Acta Pharmaceutica Sinica B 2018;8(1):64-73
CountryChina
Language:English
Abstract: Exploiting cells as vehicles combined with nanoparticles combined with therapy has attracted increasing attention in the world recently. Red blood cells, leukocytes and stem cells have been used for tumor immunotherapy, tissue regeneration and inflammatory disorders, and it is known that neutrophils can accumulate in brain lesions in many brain diseases including depression. -Acetyl Pro-Gly-Pro (PGP) peptide shows high specific binding affinity to neutrophils through the CXCR2 receptor. In this study, PGP was used to modify baicalein-loaded solid lipid nanoparticles (PGP-SLNs) to facilitate binding to neutrophils . Brain-targeted delivery to the basolateral amygdala (BLA) was demonstrated by enhanced concentration of baicalein in the BLA. An enhanced anti-depressant effect was observed and The mechanism involved inhibition of apoptosis and a decrease in lactate dehydrogenase release. Behavioral evaluation carried out with rats demonstrated that anti-depression outcomes were achieved. The results indicate that PGP-SLNs decrease immobility time, increase swimming time and climbing time and attenuate locomotion in olfactory-bulbectomized (OB) rats. In conclusion, PGP modification is a strategy for targeting the brain with a cell-nanoparticle delivery system for depression therapy.