A β-catenin/IQGAP1 regulatory feedback loop and its effects on the proliferation of colon cancer cells.
10.7507/1001-5515.201701041
- Author:
Huanji XU
1
,
2
;
Hongwei XIA
2
;
Qiulin TANG
2
;
Feng BI
1
,
3
Author Information
1. Department of Abdominal Oncology, Cancer Center, West China Hospital, Sichuan University, Chengdu 610041, P.R.China
2. Laboratory of Molecular Targeted Therapy in Oncology, West China Hospital, Sichuan University, Chengdu 610041, P.R.China.
3. Laboratory of Molecular Targeted Therapy in Oncology, West China Hospital, Sichuan University, Chengdu 610041, P.R.China.bifeng@medmail.com.cn.
- Publication Type:Journal Article
- Keywords:
IQ motif containing GTPase activating protein 1;
cell proliferation;
mitogen-activated protein kinase signaling;
β-catenin
- From:
Journal of Biomedical Engineering
2018;35(1):81-86
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this article is to study the regulatory feedback loop between β-catenin and IQ motif containing GTPase activating protein 1 (IQGAP1), as well as the effect of this regulation loop in colon cancer cell proliferation. Western blot was used to detect the expression of IQGAP1 and β-catenin after changing their expression respectively by transfection in SW1116 cells. CCK-8 cell proliferation assay was used to detect the effect of IQGAP1 involved in the proliferation of SW1116 cells promoted by β-catenin. The results of Western blot indicated that β-catenin could positively regulate IQGAP1, while IQGAP1 silencing could up-regulate β-catenin, forming a negative feedback loop. The results of CCK-8 showed that IQGAP1 silencing inhibited β-catenin-mediated proliferation in SW1116 cells. In conclusion, our research reveals a negative regulatory feedback loop between β-catenin and IQGAP1 which has a remarkable effect on the proliferation ability of colon cancer cells.
