Anti-tumor immunostimulatory effect of heat-killed tumor cells.
10.3858/emm.2008.40.1.130
- Author:
Taek Joon YOON
1
;
Ji Yeon KIM
;
Hyojeong KIM
;
Changwan HONG
;
Hyunji LEE
;
Chang Kwon LEE
;
Kwang Ho LEE
;
Seokmann HONG
;
Se Ho PARK
Author Information
1. MD Bioalpha Inc., Suwon 443-813, Korea.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
cancer vaccines;
dendritic cells;
immunotherapy, active;
interleukin 12;
mice
- MeSH:
Adjuvants, Immunologic;
Animals;
Antibodies, Neoplasm/immunology;
Antibody Specificity/immunology;
Antigens, Neoplasm/immunology;
Cancer Vaccines/*immunology;
Cell Line, Tumor;
Cell Proliferation;
Cytokines/biosynthesis;
Cytotoxicity, Immunologic/immunology;
Dendritic Cells/immunology;
*Hot Temperature;
Immunity, Cellular/immunology;
Immunization;
Immunologic Memory/immunology;
Macrophages, Peritoneal/immunology;
Mice;
Mice, Inbred BALB C;
Mice, Inbred C57BL;
Neoplasms/*immunology/*pathology;
Survival Analysis;
T-Lymphocytes, Cytotoxic/immunology
- From:Experimental & Molecular Medicine
2008;40(1):130-144
- CountryRepublic of Korea
- Language:English
-
Abstract:
As a part of our ongoing search for a safe and efficient anti-tumor vaccine, we attempted to determine whether the molecular nature of certain tumor antigens would influence immune responses against tumor cells. As compared with freeze-thawed or formaldehyde-fixed tumor antigens, heat-denatured tumor antigens elicited profound anti-tumor immune responses and greatly inhibited the growth of live tumor cells. The heat-denatured tumor antigens induced a substantial increase in the anti-tumor CTL response in the absence of any adjuvant material. This response appears to be initiated by strong activation of the antigen-presenting cells, which may recognize heat-denatured protein antigens. Upon recognition of the heat-denatured tumor antigens, macrophages and dendritic cells were found to acutely upregulate the expression of co-stimulatory molecules such as B7.2, as well as the secretion of inflammatory cytokines such as IL-12 and TNF-alpha. The results of this study indicate that heat-denatured tumor extracts might elicit protective anti-tumor adaptive immune responses and also raise the possibility that a safe and efficient adjuvant-free tumor vaccine might be developed in conjunction with a dendritic cell-based tumor vaccine.
