Effects of Irradiated Frozen Allogenic Bone and Musculoskeletal Transplant Foundation on Bone Formation in Human Fetal Osteoblasts.

Author: Ho Sang YOON ¹ ; Sung Hee PI ; Hyung Sik SHIN
Author Information

1. Department of Periodontology, School of Dentistry, Wonkwang University, Korea. pshperio@wonkwang.ac.kr
Publication Type:Original Article
Keywords: osteoblast; bone graft; allograft
MeSH: Humans
From:The Journal of the Korean Academy of Periodontology 2006;36(2):435-448
CountryRepublic of Korea
Language:Korean
Abstract: The purpose of this study was to investigate the effects of ICB(Irradiated frozen allogenic bone, Rocky Mountain Tissue Bank, USA) and MTF(Decalcified freeze-dried bone allograft, Musculoskeletal Transplant Foundation, USA) on the cell proliferation and differentiation of human fetal osteoblasts. Human fetal osteoblasts (hFOB1) were cultured with 10 ng/ml of ICB and MTF. The negatvie control group was cultured with DMSO and positive control group was cultured with BMP (2 ng/ml). MTT was performed to examine the viability of the cell, and alkaline phosphatase activity was analyzed to examine the mineralization. Calcium accumulation was also evaluated. ICB and MTF did not increase the rate of the cellular proliferation of hFOB1s while they enhanced ALP and calcium accumulation. The expression of osteocalcin (OC) and bone silaloprotein (BSP) increased in hFOB1 treated with ICB and MTF (10 ng/ml). These results suggest that ICB and MTF stimulate osteoblastic activity of the hFOB1.