Propofol protects against oxidative-stress-induced COS-7 cell apoptosis by inducing autophagy.
10.17245/jdapm.2017.17.1.37
- Author:
Ji Young YOON
1
;
Chul Woo BAEK
;
Eun Jung KIM
;
Bong Soo PARK
;
Su Bin YU
;
Ji Uk YOON
;
Eok Nyun KIM
Author Information
1. Department of Dental Anesthesia and Pain Medicine, School of Dentistry, Pusan National University, Dental Research Institute, Yangsan, Republic of Korea. chemfrie@naver.com
- Publication Type:Original Article
- Keywords:
Autophagy;
COS-7 Cells;
Oxidative Stress;
Propofol
- MeSH:
Animals;
Apoptosis*;
Autophagy*;
Blotting, Western;
Cell Survival;
COS Cells*;
Hydrogen Peroxide;
Methods;
Microscopy, Fluorescence;
Oxidative Stress;
Propofol*;
Reactive Oxygen Species
- From:Journal of Dental Anesthesia and Pain Medicine
2017;17(1):37-46
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: In oxidative stress, reactive oxygen species (ROS) production contributes to cellular dysfunction and initiates the apoptotic cascade. Autophagy is considered the mechanism that decreases ROS concentration and oxidative damage. Propofol shows antioxidant properties, but the mechanisms underlying the effect of propofol preconditioning (PPC) on oxidative injury remain unclear. Therefore, we investigated whether PPC protects against cell damage from hydrogen peroxide (H₂O₂)-induced oxidative stress and influences cellular autophagy. METHOD: COS-7 cells were randomly divided into the following groups: control, cells were incubated in normoxia (5% CO₂, 21% O₂, and 74% N₂) for 24 h without propofol; H₂O₂, cells were exposed to H₂O₂ (400 µM) for 2 h; PPC + H₂O₂, cells pretreated with propofol were exposed to H₂O₂; and 3-methyladenine (3-MA) + PPC + H₂O₂, cells pretreated with 3-MA (1 mM) for 1 h and propofol were exposed to H₂O₂. Cell viability was determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide thiazolyl blue (MTT) reduction. Apoptosis was determined using Hoechst 33342 staining and fluorescence microscopy. The relationship between PPC and autophagy was detected using western blot analysis. RESULTS: Cell viability decreased more significantly in the H₂O₂ group than in the control group, but it was improved by PPC (100 µM). Pretreatment with propofol effectively decreased H₂O₂-induced COS-7 cell apoptosis. However, pretreatment with 3-MA inhibited the protective effect of propofol during apoptosis. Western blot analysis showed that the level of autophagy-related proteins was higher in the PPC + H₂O₂ group than that in the H2O2 group. CONCLUSION: PPC has a protective effect on H₂O₂-induced COS-7 cell apoptosis, which is mediated by autophagy activation.
