Ex vivo Expansion of Human Natural Killer Cells from Blood Retained in a Disposable Platelet Apheresis Set.
- Author:
Dong Jun SHIN
1
;
Duck CHO
;
Youn Young JANG
;
Ji Yun PARK
;
Ju Sun KIM
;
Sang Ki KIM
;
Je Jung LEE
;
Min Joong JANG
;
Myung Geun SHIN
;
Dong Wook RYANG
Author Information
1. Research Center for Cancer Immunotherapy, Chonnam National University Hwasun Hospital, Hwasun, Korea. dcho@chonnam.ac.kr
- Publication Type:Original Article
- Keywords:
Natural killer cell;
Ex vivo expansion;
Apheresis set
- MeSH:
4-1BB Ligand;
Blood Component Removal;
Blood Platelets;
Humans;
Immunotherapy;
Interleukin-15;
Interleukin-2;
K562 Cells;
Killer Cells, Natural;
Tissue Donors
- From:Korean Journal of Blood Transfusion
2012;23(1):28-37
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Natural killer cells expanded from human peripheral blood (PB) have been used in cancer immunotherapy research. Although most research teams have access to human PB, it is necessary to find a source of blood that can be easily obtained. We have tested the possibility of using blood retained in a disposable platelet apheresis set as an alternative source, with special interest in expansion of NK cells for use in cancer immunotherapy research. METHODS: For expansion of NK cells, peripheral blood mononuclear cells (PBMCs) were isolated from an MCS+ platelet apheresis kit (Haemonetics, Braintree, USA) and PB from the same donor (n=7) and co-cultured with 100-Gy gamma ray-irradiated K562 cells expressing the 4-1BB ligand and membrane-bound IL-15 for three weeks in RPMI1640 medium in the presence of IL-2 and IL-15. Cytotoxicity was measured using WST-1 at 1:1, 2:1, and 4:1 effector-to-target (E:T) ratios for a period of four hours. RESULTS: Mean rate of expansion of NK cells was 1,097-fold and their purity was 94.4% from blood retained in a disposable platelet apheresis set; mean rate of expansion of NK cells was 953-fold and their purity was 92.0% from PB after a period of three weeks. No differences in cytotoxicity against K562, 697, Raji, and RPMI8226 were observed between NK cells expanded from two blood sources. CONCLUSION: Blood retained in a disposable platelet apheresis set is a useful and convenient source for expansion of NK cells for use in cancer immunotherapy research.
