Resveratrol inhibits foam cell formation via NADPH oxidase 1-mediated reactive oxygen species and monocyte chemotactic protein-1.
10.3858/emm.2009.41.3.020
- Author:
Dae Weon PARK
1
;
Kheewoong BAEK
;
Jae Ryong KIM
;
Jae Jin LEE
;
Sang Ho RYU
;
Byung Rho CHIN
;
Suk Hwan BAEK
Author Information
1. Aging-Associated Vascular Disease Research Center. Department of Biochemistry and Molecular Biology, Yeungnam University Daegu 705-717, Korea. sbaek@med.yu.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
atherosclerosis;
CCL2 protein;
foam cells;
FoxO3a protein;
NADPH oxidase 1;
reactive oxygen species;
resveratrol
- MeSH:
Antioxidants/*pharmacology;
Cells, Cultured;
Chemokine CCL2/genetics/*metabolism;
Enzyme Activation/drug effects;
Foam Cells/*drug effects/physiology;
Forkhead Transcription Factors/metabolism;
Humans;
Lipopolysaccharides/pharmacology;
NADH, NADPH Oxidoreductases/genetics/*metabolism;
Proto-Oncogene Proteins c-akt/metabolism;
RNA, Messenger/metabolism;
Reactive Oxygen Species/*metabolism;
Signal Transduction;
Stilbenes/*pharmacology
- From:Experimental & Molecular Medicine
2009;41(3):171-179
- CountryRepublic of Korea
- Language:English
-
Abstract:
Resveratrol is a polyphenolic compound in red wine that has anti-oxidant and cardioprotective effects in animal models. Reactive oxygen species (ROS) and monocyte chemotactic protein-1 (MCP-1) play key roles in foam cell formation and atherosclerosis. We studied LPS-mediated foam cell formation and the effect of resveratrol. Resveratrol pretreatment strongly suppressed LPS-induced foam cell formation. To determine if resveratrol affected the expression of genes that control ROS generation in macrophages, NADPH oxidase 1 (Nox1) was measured. Resveratrol treatment of macrophages inhibited LPS-induced Nox1 expression as well as ROS generation, and also suppressed LPS-induced MCP-1 mRNA and protein expression. We investigated the upstream targets of Nox1 and MCP-1 expression and found that Akt-forkhead transcription factors of the O class (FoxO3a) is an important signaling pathway that regulates both genes. These inhibitory effects of resveratrol on Nox1 expression and MCP-1 production may target to the Akt and FoxO3a signaling pathways.
