Langerhans cell protein 1 (LCP1) binds to PNUTS in the nucleus: implications for this complex in transcriptional regulation.
10.3858/emm.2009.41.3.022
- Author:
Shin Jeong LEE
1
;
Jun Ki LEE
;
Yong Sun MAENG
;
Young Myeong KIM
;
Young Guen KWON
Author Information
1. Department of Biochemistry, College of Life Science and Biotechnology, Yonsei University, Seoul 120-752, Korea. ygkwon@yonsei.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
PPP1R10 protein, human;
protein interaction mapping;
transcription factors;
two-hybrid system techniques
- MeSH:
Amino Acid Sequence;
Cell Line;
Cell Nucleus/*metabolism;
DNA-Binding Proteins/*metabolism;
HMGB Proteins/*metabolism;
Humans;
Molecular Sequence Data;
Nuclear Proteins/*metabolism;
Protein Binding;
Protein Interaction Mapping;
RNA-Binding Proteins/*metabolism;
Transcriptional Activation;
Two-Hybrid System Techniques
- From:Experimental & Molecular Medicine
2009;41(3):189-200
- CountryRepublic of Korea
- Language:English
-
Abstract:
Protein phosphatase-1 (PP1) nuclear targeting subunit (PNUTS), also called PP1R10, p99, or CAT 53 was originally isolated as a mammalian nuclear PP1-binding protein. In this study, we performed yeast two-hybrid screens to identify PNUTS-interacting proteins. Here, we report that LCP1 (epidermal Langerhans cell protein 1), a novel member of the HMG-box protein family, binds tightly to PNUTS. Co-immunoprecipitation of deletion constructs revealed that the C-terminus of LCP1 is sufficient for the interaction with an N-terminal region of PNUTS that is distinct from its PP1-binding domain. Furthermore, immunofluorescence studies showed that a subpopulation of LCP1 co-localizes with PNUTS in nuclear speckles. Importantly, we found that the N-terminus of LCP1 has a strong trans-activation activity in a GAL4-based heterologous transcription assay. The transcriptional activity of LCP1 is markedly suppressed by its interaction with PNUTS, in a PP1-independent manner. These findings suggest that the coordinated spatial and temporal regulation of LCP1 and PNUTS may be a novel mechanism to control the expression of genes that are critical for certain physiological and pathological processes.
