Improvement of the osteogenic potential of ErhBMP-2-/EGCG-coated biphasic calcium phosphate bone substitute: in vitro and in vivo activity
10.5051/jpis.2019.49.2.114
- Author:
Jae ho HWANG
1
;
Seunghan OH
;
Sungtae KIM
Author Information
1. Department of Periodontology, Dental Research Institute, Seoul National University School of Dentistry, Seoul, Korea. kst72@snu.ac.kr
- Publication Type:Original Article
- Keywords:
Biphasic calcium phosphate;
Bone morphogenetic protein 2;
Bone substitute;
epigallocatechin-3-gallate
- MeSH:
Animals;
Bone Morphogenetic Protein 2;
Bone Substitutes;
Calcium;
Cell Survival;
Escherichia;
Humans;
In Vitro Techniques;
Male;
Maxillary Sinus;
Membranes;
Miners;
Osteoblasts;
Rabbits;
Transplants
- From:Journal of Periodontal & Implant Science
2019;49(2):114-126
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: The aim of this study was to evaluate the enhancement of osteogenic potential of biphasic calcium phosphate (BCP) bone substitute coated with Escherichia coli-derived recombinant human bone morphogenetic protein-2 (ErhBMP-2) and epigallocatechin-3-gallate (EGCG). METHODS: The cell viability, differentiation, and mineralization of osteoblasts was tested with ErhBMP-2-/EGCG solution. Coated BCP surfaces were also investigated. Standardized, 6-mm diameter defects were created bilaterally on the maxillary sinus of 10 male New Zealand white rabbits. After removal of the bony windows and elevation of sinus membranes, ErhBMP-2-/EGCG-coated BCP was applied on one defect in the test group. BCP was applied on the other defect to form the control group. The animals were sacrificed at 4 or 8 weeks after surgery. Histologic and histometric analyses of the augmented graft and surrounding tissue were performed. RESULTS: The 4-week and 8-week test groups showed more new bone (%) than the corresponding control groups (P<0.05). The 8-week test group showed more new bone (%) than the 4-week test group (P<0.05). CONCLUSIONS: ErhBMP-2-/EGCG-coated BCP was effective as a bone graft material, showing enhanced osteogenic potential and minimal side effects in a rabbit sinus augmentation model.
