Adjunctive hyperbaric oxygen therapy for irradiated rat calvarial defects
- Author:
Heesuk AN
1
;
Jung Tae LEE
;
Seo Eun OH
;
Kyeong mee PARK
;
Kyung Seok HU
;
Sungtae KIM
;
Moon Kyu CHUNG
Author Information
- Publication Type:Original Article
- Keywords: Biphasic calcium phosphate; Fibroblast growth factor-2; Hyperbaric oxygen therapy; Radiotherapy
- MeSH: Animals; Bone Regeneration; Calcium; Fibroblast Growth Factor 2; Hyaluronic Acid; Hyperbaric Oxygenation; Membranes; Osteogenesis; Oxygen; Parietal Bone; Radiotherapy; Rats; Skull; Transplants
- From:Journal of Periodontal & Implant Science 2019;49(1):2-13
- CountryRepublic of Korea
- Language:English
- Abstract: PURPOSE: The aim of this study was to conduct a histologic evaluation of irradiated calvarial defects in rats 4 weeks after applying fibroblast growth factor-2 (FGF-2) with hyaluronan or biphasic calcium phosphate (BCP) block in the presence or absence of adjunctive hyperbaric oxygen (HBO) therapy. METHODS: Twenty rats were divided into HBO and non-HBO (NHBO) groups, each of which was divided into FGF-2 and BCP-block subgroups according to the grafted material. Localized radiation with a single 12-Gy dose was applied to the calvaria of rats to simulate radiotherapy. Four weeks after applying this radiation, 2 symmetrical circular defects with a diameter of 6 mm were created in the parietal bones of each animal. The right-side defect was filled with the materials mentioned above and the left-side defect was not filled (as a control). All defects were covered with a resorbable barrier membrane. During 4 weeks of healing, 1 hour of HBO therapy was applied to the rats in the HBO groups 5 times a week. The rats were then killed, and the calvarial specimens were harvested for radiographic and histologic analyses. RESULTS: New bone formation was greatest in the FGF-2 subgroup, and improvement was not found in the BCP subgroup. HBO seemed to have a minimal effect on new bone formation. There was tendency for more angiogenesis in the HBO groups than the NHBO groups, but the group with HBO and FGF-2 did not show significantly better outcomes than the HBO-only group or the NHBO group with FGF-2. CONCLUSIONS: HBO exerted beneficial effects on angiogenesis in calvarial defects of irradiated rats over a 4-week healing period, but it appeared to have minimal effects on bone regeneration. FGF-2 seemed to enhance new bone formation and angiogenesis, but its efficacy appeared to be reduced when HBO was applied.
